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A Highly Sensitive Immunoassay for Determination of Immune Response to SARS-CoV-2 in Capillary Blood Samples.
Sánchez, Belén G; Bort, Alicia; Mora-Rodríguez, José María; Díaz-Yuste, Alba; Gasalla, José Manuel; Sánchez-Chapado, Manuel; Sebastián-Martín, Alba; Díaz-Laviada, Inés.
  • Sánchez BG; Biochemistry and Molecular Biology Unit, Department of Systems Biology, School of Medicine and Health Sciences, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
  • Bort A; Department of Comparative Medicine, School of Medicine, Yale University, New Haven, CT 06519, USA.
  • Mora-Rodríguez JM; Biochemistry and Molecular Biology Unit, Department of Systems Biology, School of Medicine and Health Sciences, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
  • Díaz-Yuste A; Biochemistry and Molecular Biology Unit, Department of Systems Biology, School of Medicine and Health Sciences, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
  • Gasalla JM; Biochemistry and Molecular Biology Unit, Department of Systems Biology, School of Medicine and Health Sciences, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
  • Sánchez-Chapado M; Clinical Biochemistry Service, Príncipe de Asturias Hospital, 28805 Alcalá de Henares, Madrid, Spain.
  • Sebastián-Martín A; Department of Urology, Príncipe de Asturias Hospital, 28805 Alcalá de Henares, Madrid, Spain.
  • Díaz-Laviada I; Department of Surgery, Medical and Social Sciences, School of Medicine and Health Sciences, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
Biomedicines ; 10(11)2022 Nov 11.
Article in English | MEDLINE | ID: covidwho-2109932
ABSTRACT
Throughout the pandemic, serological assays have been revealed as crucial for detecting previous exposures to the virus and determining the timing of antibody maintenance after vaccination or natural infection. This study aimed to develop an optimized enzyme-linked immunosorbent assay (ELISA)-based serology, which could be used in case of reagent shortages, such as that occurred in the beginning of this health emergency. As a result, we present a high-sensitive immunoassay for the determination of IgG levels in venous serum samples, using 2 µg/mL antigen (receptor-binding domain of the spike protein S1) for coating the plate and utilizing human samples at a dilution 11000. This method showed non-inferiority features versus a commercial kit, is less expensive, and has a higher spectrophotometric range that allows for a better quantification of the antibody titers. The optical density values before and after heating venous serum samples at 56 °C during 30 min was quite similar, showing that heat inactivation can be used to reduce the biohazardous risks while handling samples. Furthermore, we show that finger-stick capillary blood samples can also serve as a suitable source for IgG detection, bypassing the need for serum isolation and being suitable for point-of-care application (Pearson's coefficient correlation with capillary serum was 0.95, being statistically significant).
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Experimental Studies / Prognostic study Topics: Vaccines Language: English Year: 2022 Document Type: Article Affiliation country: Biomedicines10112897

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Experimental Studies / Prognostic study Topics: Vaccines Language: English Year: 2022 Document Type: Article Affiliation country: Biomedicines10112897