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[Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies].
Xue, Xiongyan; Zhu, Changlin; Huang, Shaozhen; Pan, Lianhua; Xu, Jianhua; Li, Weixuan.
  • Xue X; Clinical Laboratory, Foshan First People's Hospital, Foshan 528000, China.
  • Zhu C; Clinical Laboratory, Foshan First People's Hospital, Foshan 528000, China.
  • Huang S; Clinical Laboratory, Foshan First People's Hospital, Foshan 528000, China.
  • Pan L; Clinical Laboratory, Foshan First People's Hospital, Foshan 528000, China.
  • Xu J; Medical Laboratory Center, Shunde Hospital of Guangzhou University of Chinese Medicine, Foshan 528333, China.
  • Li W; Clinical Laboratory, Foshan First People's Hospital, Foshan 528000, China.
Nan Fang Yi Ke Da Xue Xue Bao ; 40(3): 316-320, 2020 Mar 30.
Article in Chinese | MEDLINE | ID: covidwho-211001
ABSTRACT

OBJECTIVE:

To evaluate the effects of heat inactivation of blood samples at 56℃ for 30 min on the results of SARS-CoV-2 antibody detection using different methods.

METHODS:

This retrospective study was conducted in 11 patients with established diagnosis of COVID-19 and 10 patients with diseases other than COVID- 19 in our hospital. We collected samples of serum, plasma and whole blood from each patient between February, 12 and 18, 2020, and with a double- blind design, the samples were examined for SARS-CoV-2 antibodies before and after heat inactivation at 56 ℃ for 30 min. In all the samples, the total SARS-CoV-2 antibodies were detected using immunochromatography, and the IgM antibodies were detected using fluorescence immunochromatography; the IgM and IgG antibodies in the serum and plasma samples detected with chemiluminescence immunoassay. We compared the detection results and analyzed the correlation of semi-quantitative detection results of IgM and IgG antibodies before and after heat inactivation of the samples.

RESULTS:

With immuno-chromatography, the coincidence rate of the total SARS-CoV-2 antibody detection before and after heat inactivation of the serum and plasma samples was 90.0% in COVID-19 cases and 100.0% in the negative cases, resulting in a total coincidence rate 95.2%; for the whole blood samples, the total coincidence rates of the total SARS-CoV-2 antibodies were 100.0%. For detection of IgM antibodies in the serum, plasma and whole blood samples using fluorescence immunochromatography, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were 100.0%, 0 and 47.6%, respectively. For detection of serum IgM and IgG antibodies and plasma IgG antibodies with chemiluminescence immunoassay, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were all 100.0%, and the total coincidence rate of plasma IgM antibodies was 95.2%. Pearson correlation analysis of the semi-quantitative results of IgM and IgG detection in the serum and plasma samples showed a correlation coefficient of 0.9999 (95%CI 0.9998-1.000, P < 0.001) between the results before and after sample inactivation.

CONCLUSIONS:

Heat inactivation of blood samples at 56 ℃ for 30 min does not obviously affect the results of immunochromatography and chemiluminescent immunoassay for detection of SARS-COV-2 antibodies but can reduce the risk of infection for the operators. Heat-inactivated samples can not be used in fluorescence immunochromatography for SARS-CoV-2 antibody detection.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Limits: Humans Language: Chinese Journal: Nan Fang Yi Ke Da Xue Xue Bao Year: 2020 Document Type: Article Affiliation country: J.issn.1673-4254.2020.03.03

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Limits: Humans Language: Chinese Journal: Nan Fang Yi Ke Da Xue Xue Bao Year: 2020 Document Type: Article Affiliation country: J.issn.1673-4254.2020.03.03