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Direct self-sampled gargle water LAMP as a screening method for the detection of SARS-CoV-2 infections
Eesti Arst ; 101(Supplement 4):25, 2022.
Article in English | EMBASE | ID: covidwho-2111899
ABSTRACT
The COVID-19 pandemic accelerated the development of diagnostic alternatives. One of the alternatives to RT-PCR (the gold standard for SARS-CoV-2 diagnostics) is direct reverse transcription loop-mediated isothermal amplification (direct RT-LAMP or just LAMP). A LAMP reaction can be completed in about 30 min. and requires no temperature cycling. Furthermore, LAMP allows for various read-out options, such as fluorescence, electrochemical, or probe-based methods combined with real-time or endpoint detection. In this study, we assessed the viability of self-sampled gargle water direct RT-LAMP (LAMP) for detecting SARS-CoV-2 infections by estimating its sensitivity with respect to the gold standard indirect RT-PCR of paired oro-naso-pharyngeal swab samples. We also assessed the impact of symptom onset to test time (STT) - i.e., symptom days at sampling, on LAMP. In addition, we appraised the viability of gargle water self-sampling versus oro-nasopharyngeal swab sampling, by comparing paired indirect RT-PCR results. 202 oro-nasopharyngeal swab and paired self-sampled gargle water samples were collected from hospital patients with COVID-19-associated symptoms. LAMP, indirect and direct RT-PCR were performed on all gargle water samples, and indirect RT-PCR was performed on all oro-nasopharyngeal samples. LAMP presented a sensitivity of 80.8% (95% CI 70.8-90.8%) for sample pairs with sub-25 Ct oro-nasopharyngeal indirect RT-PCR results, and 77.6% (66.2-89.1%) sensitivity for sub-30 Ct samples with STT <= 7 days. STT, independently of Ct value, correlated negatively with LAMP performance. 80.7% agreement was observed between gargle water and oro-nasopharyngeal indirect RT-PCR results. In conclusion, LAMP presents an acceptable sensitivity for low Ct and low STT samples. Gargle water may be considered as a viable sampling method, and LAMP as a screening method, especially for symptomatic persons with low STT values.
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Collection: Databases of international organizations Database: EMBASE Type of study: Diagnostic study Language: English Journal: Eesti Arst Year: 2022 Document Type: Article

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Collection: Databases of international organizations Database: EMBASE Type of study: Diagnostic study Language: English Journal: Eesti Arst Year: 2022 Document Type: Article