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Preparation of antibodies against the spike glycoprotein of SARS -CoV- 2 and establishment of a double antibody sandwich ELISA for detection of the spike glycoprotein
Chinese Journal of Virology ; 38(1):14-20, 2022.
Article in Chinese | GIM | ID: covidwho-2115896
ABSTRACT
The coronavirus disease 2019 (COVID-19) pandemic poses a serious threat to human life and health. To establish a method for quantitative detection of detection of the spike glycoprotein of severe acute respiratory syndrome coronavirus'Z (SARS-CoV-Z) in vaccines. Goat anti-SARS-CoV-Z polyclonal antibody and mouse anti -SARS -CoV-Z spike glycoprotein monoclonal antibody were prepared to establish a double antibody sandwich enzymerlinked immunosorbent assay (ELISA) for detection of the spike glycoprotein. The ELISA system was optimized and the linear range, sensitivity, specificity, repeatability and coincidence rate were tested. The linear range was 1-64 U and correlation coefficient (R2) > 0.99. There was no reaction with the nucleocapsid protein, Vero-cell debris or influenza Viruses, etc, indicating the high specificity of our method. The sensitivity was 92.1% and the variations in intra- and inter' assay repeatability were 2.5%-11.7% and 1.3% -14.8%, respectively. The samples showed a coincidence rate Of 96.7% with known background. Our method had high specificity, sensitivity, stability and accuracy, and could be used for determination of spike - glycoprotein antigen content in vaccine.
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Full text: Available Collection: Databases of international organizations Database: GIM Language: Chinese Journal: Chinese Journal of Virology Year: 2022 Document Type: Article

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Full text: Available Collection: Databases of international organizations Database: GIM Language: Chinese Journal: Chinese Journal of Virology Year: 2022 Document Type: Article