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Sample-to-Answer Immuno-Magnetic Assay Using Thermally Responsive Alkane Partitions.
Everitt, Micaela L; Boegner, David J; White, Ian M.
  • Everitt ML; Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
  • Boegner DJ; Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
  • White IM; Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Biosensors (Basel) ; 12(11)2022 Nov 17.
Article in English | MEDLINE | ID: covidwho-2116030
ABSTRACT
To combat pandemics, there is a need for rapid point-of-care diagnostics to identify infected patients and to track the spread of the disease. While recent progress has been made in response to COVID-19, there continues to be a need for point-of-care diagnostics capable of detecting biomarkers-such as antibodies-in whole blood. We have recently reported the development of thermally responsive alkane partitions (TRAPs) for the automation of point-of-care immuno-magnetic assays. Here, we demonstrate the use of TRAPs to enable sample-to-answer detection of antibodies against the SARS-CoV-2 virus in whole blood samples. We report a limit of detection of 84 pg/mL, well below the clinically relevant threshold. We anticipate that the TRAP-enabled sample-to-answer immunoassay can be used to track the progression of future pandemics, leading to a more informed and robust clinical and societal response.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Alkanes / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Bios12111030

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Alkanes / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Bios12111030