Your browser doesn't support javascript.
Novel RT-PCR Using Sugar Chain-Immobilized Gold-Nanoparticles Correlates Patients' Symptoms: The Follow-Up Study of COVID-19 Hospitalized Patients.
Kajiya, Takashi; Sawayama, Hayate; Arima, Eriko; Okamoto, Mika; Baba, Masanori; Toyama, Masaaki; Okuya, Kosuke; Ozawa, Makoto; Atsuchi, Nobuhiko; Nishi, Junichiro; Suda, Yasuo.
  • Kajiya T; Clinical Research Center, Tenyoukai Central Hospital, 6-7 Izumi-cho, Kagoshima 892-0822, Japan.
  • Sawayama H; Laboratory of Collaborational Research for Glyco-Nanotechnology, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Kohrimoto, Kagoshima 890-0065, Japan.
  • Arima E; Laboratory of Collaborational Research for Glyco-Nanotechnology, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Kohrimoto, Kagoshima 890-0065, Japan.
  • Okamoto M; Center for Advanced Science Research and Promotion, Kagoshima University, Kagoshima 890-0065, Japan.
  • Baba M; Center for Advanced Science Research and Promotion, Kagoshima University, Kagoshima 890-0065, Japan.
  • Toyama M; Joint Research Center for Human Retrovirus Infection, Kagoshima University, Kagoshima 890-8544, Japan.
  • Okuya K; Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
  • Ozawa M; Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
  • Atsuchi N; Clinical Research Center, Tenyoukai Central Hospital, 6-7 Izumi-cho, Kagoshima 892-0822, Japan.
  • Nishi J; Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan.
  • Suda Y; Laboratory of Collaborational Research for Glyco-Nanotechnology, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Kohrimoto, Kagoshima 890-0065, Japan.
Viruses ; 14(11)2022 Nov 21.
Article in English | MEDLINE | ID: covidwho-2116086
ABSTRACT

Background:

The transmissible capacity and toxicity of SARS-CoV-2 variants are continually changing. We report here the follow-up study of hospitalized COVID-19 patients from 2020 to 2022. It is known that the PCR diagnosis for hospitalized patients sometimes causes confusion because of the incompatibility between their diagnosis and symptoms. We applied our sugar chain-immobilized gold-nanoparticles for the extraction and partial purification of RNA from specimens for quantitative RT-PCR assay and evaluated whether the results correlate with patients' symptoms. Methods and

Results:

Saliva specimens were taken from hospitalized patients with mild or moderate symptoms every early morning. At the time of RT-PCR diagnosis, two methods for the extraction and partial purification of RNA from the specimen were performed a commonly used Boom (Qiagen) method and our original sugar chain-immobilized gold nanoparticle (SGNP) method. For symptoms, body temperature and oxygen saturation (SpO2) of patients were monitored every 4 h.

Conclusions:

It was clear that patients infected with the Delta variant needed more time to recover than those with the Omicron variant, and that the SGNP method showed more realistic correlation with the symptoms of patients compared with the common Qiagen method.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Metal Nanoparticles / COVID-19 Type of study: Cohort study / Diagnostic study / Experimental Studies / Prognostic study Topics: Variants Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: V14112577

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Metal Nanoparticles / COVID-19 Type of study: Cohort study / Diagnostic study / Experimental Studies / Prognostic study Topics: Variants Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: V14112577