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SARS-CoV-2 variant detection from wastewater: rapid spread of B.1.1.7 lineage in Hungary.
Róka, Eszter; Déri, Dániel; Khayer, Bernadett; Kis, Zoltán; Schuler, Eszter; Magyar, Nóra; Pályi, Bernadett; Pándics, Tamás; Vargha, Márta.
  • Róka E; Department of Public Health Laboratory, National Public Health Center, Albert Flórián út 2-6., H-1097 Budapest, Hungary E-mail: vargha.marta@nnk.gov.hu.
  • Déri D; National Biosafety Laboratory, Division of Microbiological Reference Laboratories, National Public Health Center, Albert Flórián út 2-6., H-1097 Budapest, Hungary.
  • Khayer B; Department of Public Health Laboratory, National Public Health Center, Albert Flórián út 2-6., H-1097 Budapest, Hungary E-mail: vargha.marta@nnk.gov.hu.
  • Kis Z; National Biosafety Laboratory, Division of Microbiological Reference Laboratories, National Public Health Center, Albert Flórián út 2-6., H-1097 Budapest, Hungary.
  • Schuler E; Department of Public Health Laboratory, National Public Health Center, Albert Flórián út 2-6., H-1097 Budapest, Hungary E-mail: vargha.marta@nnk.gov.hu.
  • Magyar N; National Biosafety Laboratory, Division of Microbiological Reference Laboratories, National Public Health Center, Albert Flórián út 2-6., H-1097 Budapest, Hungary.
  • Pályi B; National Biosafety Laboratory, Division of Microbiological Reference Laboratories, National Public Health Center, Albert Flórián út 2-6., H-1097 Budapest, Hungary.
  • Pándics T; Department of Public Health Laboratory, National Public Health Center, Albert Flórián út 2-6., H-1097 Budapest, Hungary E-mail: vargha.marta@nnk.gov.hu.
  • Vargha M; Department of Public Health Laboratory, National Public Health Center, Albert Flórián út 2-6., H-1097 Budapest, Hungary E-mail: vargha.marta@nnk.gov.hu.
J Water Health ; 20(2): 277-286, 2022 Feb.
Article in English | MEDLINE | ID: covidwho-2117661
ABSTRACT
Wastewater-based epidemiology (WBE) is a recognised tool for tracking community transmission of COVID-19. From the second half of 2020, the emergence of new, highly infective, more pathogenic or vaccine-escape SARS-CoV-2 variants is the major public health concern. Variant analysis in sewage might assist the early detection of new mutations. Weekly raw sewage samples from 22 wastewater treatment plants (WWTPs) in Hungary (representing 40% of the population) were analysed between December 2020 and March 2021 for signature mutations N501Y and del H69/V70 of B.1.1.7 lineage by melting point genotyping and RT-digital droplet PCR (RT-ddPCR). The latter method proved to be more efficient in parallel detection of different variants and also provides quantitative information. Wastewater surveillance indicated that the B.1.1.7 variant first emerged in Budapest in early January 2021 and rapidly became dominant in the entire country. Results are in close agreement with the available clinical data (Pearson's correlation coefficient, R = 0.9153). RT-ddPCR was confirmed to be a reliable tool for tracking emerging variant ratios in wastewaters. It is a rapid and cost-effective method compared to whole-genome sequencing, but only applicable for the detection of known mutations. Efficient variant surveillance might require the combination of multiple methods.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Observational study / Prognostic study Topics: Vaccines / Variants Limits: Humans Country/Region as subject: Europa Language: English Journal: J Water Health Journal subject: Environmental Health Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Observational study / Prognostic study Topics: Vaccines / Variants Limits: Humans Country/Region as subject: Europa Language: English Journal: J Water Health Journal subject: Environmental Health Year: 2022 Document Type: Article