Your browser doesn't support javascript.
Cross-reactive SARS-CoV-2 epitope targeted across donors informs immunogen design.
Hauser, Blake M; Feldman, Jared; Sangesland, Maya; Ronsard, Larance; St Denis, Kerri J; Sheehan, Maegan L; Cao, Yi; Boucau, Julie; Windsor, Ian W; Cheng, Agnes H; Vu, Mya L; Cardoso, Marcella R; Kannegieter, Ty; Balazs, Alejandro B; Lingwood, Daniel; Garcia-Beltran, Wilfredo F; Schmidt, Aaron G.
  • Hauser BM; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Feldman J; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Sangesland M; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Ronsard L; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • St Denis KJ; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Sheehan ML; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Cao Y; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Boucau J; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Windsor IW; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA; Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Laboratory of Molecular Medicine, Boston Childre
  • Cheng AH; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Vu ML; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Cardoso MR; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Kannegieter T; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Balazs AB; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Lingwood D; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Garcia-Beltran WF; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA; Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA.
  • Schmidt AG; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA; Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: aschmidt@crystal.harvard.edu.
Cell Rep Med ; : 100834, 2022 Nov 15.
Article in English | MEDLINE | ID: covidwho-2120103
ABSTRACT
The emergence of the antigenically distinct and highly transmissible Omicron variant highlights the possibility of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immune escape due to viral evolution. This continued evolution, along with the possible introduction of new sarbecoviruses from zoonotic reservoirs, may evade host immunity elicited by current SARS-CoV-2 vaccines. Identifying cross-reactive antibodies and defining their epitope(s) can provide templates for rational immunogen design strategies for next-generation vaccines. Here, we characterize the receptor-binding-domain-directed, cross-reactive humoral repertoire across 10 human vaccinated donors. We identify cross-reactive antibodies from diverse gene rearrangements targeting two conserved receptor-binding domain epitopes. An engineered immunogen enriches antibody responses to one of these conserved epitopes in mice with pre-existing SARS-CoV-2 immunity; elicited responses neutralize SARS-CoV-2, variants, and related sarbecoviruses. These data show how immune focusing to a conserved epitope targeted by human cross-reactive antibodies may guide pan-sarbecovirus vaccine development, providing a template for identifying such epitopes and translating to immunogen design.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Type of study: Randomized controlled trials Topics: Vaccines / Variants Language: English Journal: Cell Rep Med Year: 2022 Document Type: Article Affiliation country: J.xcrm.2022.100834

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Type of study: Randomized controlled trials Topics: Vaccines / Variants Language: English Journal: Cell Rep Med Year: 2022 Document Type: Article Affiliation country: J.xcrm.2022.100834