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Single-walled carbon nanotubes-based RNA protection and extraction improves RT-qPCR sensitivity for SARS-CoV-2 detection.
Li, Yong; Han, Xiangning; Mu, Xiaofeng; Wang, Ye; Shi, Chao; Ma, Cuiping.
  • Li Y; Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, 266042, Qingdao, China.
  • Han X; Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, 266042, Qingdao, China.
  • Mu X; Clinical Laboratory, Qingdao Central Hospital, The Second Affiliated Hospital of Medical College of Qingdao University, 266042, Qingdao, China.
  • Wang Y; Clinical Laboratory, Qingdao Central Hospital, The Second Affiliated Hospital of Medical College of Qingdao University, 266042, Qingdao, China.
  • Shi C; Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences, Department of Pathogenic Biology, School of Basic Medicine, the Clinical Laboratory Department of the Affiliated Hospital of Qingdao University, Qingdao University, 266071, Qingdao, Ch
  • Ma C; Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, 266042, Qingdao, China. Electronic address: mcp169@163.com.
Anal Chim Acta ; 1238: 340639, 2023 Jan 15.
Article in English | MEDLINE | ID: covidwho-2120191
ABSTRACT
The false-negative result of nucleic acid testing is an important cause of continued spread of COVID-19, while SARS-CoV-2 RNA degradation during transportation and nucleic acid extraction can lead to false-negative results. Here, we investigated that single-walled carbon nanotubes (SCNTs) could protect RNA from degradation for at least 4 days at room temperature. By constructing magnetism-functionalized SCNTs (MSCNTs), we developed a method that enabled protection and simple extraction of SARS-CoV-2 RNA, and the RNA-bound MSCNTs can be directly used for reverse transcription polymerase chain reaction (RT-qPCR) detection. The experimental results showed that 1 µg of MSCNTs adsorbed up to 24 ng of RNA. Notably, the MSCNTs-based method for extracting SARS-CoV-2 RNA from simulated nasopharyngeal swabs and saliva samples with mean recovery rates of 103% and 106% improved the sensitivity of RT-qPCR detection by 8-32 fold in comparison to current common methods. This improvement was largely attributable to the protection of RNA, enabling increased RNA load for downstream assays.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acids / Nanotubes, Carbon / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Anal Chim Acta Year: 2023 Document Type: Article Affiliation country: J.aca.2022.340639

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acids / Nanotubes, Carbon / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Anal Chim Acta Year: 2023 Document Type: Article Affiliation country: J.aca.2022.340639