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An electrochemical paper-based hydrogel immunosensor to monitor serum cytokine for predicting the severity of COVID-19 patients.
Shi, Dongmin; Zhang, Chiye; Li, Xiaoyuan; Yuan, Jie.
  • Shi D; Department of Electronic & Computer Engineering, The Hong Kong University of Science and Technology, Hong Kong Special Administrative Region of China; Individualized Interdisciplinary Program (Microelectronics), The Hong Kong University of Science and Technology, Hong Kong Special Administrative
  • Zhang C; Department of Electronic & Computer Engineering, The Hong Kong University of Science and Technology, Hong Kong Special Administrative Region of China.
  • Li X; Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong Special Administrative Region of China.
  • Yuan J; Department of Electronic & Computer Engineering, The Hong Kong University of Science and Technology, Hong Kong Special Administrative Region of China.
Biosens Bioelectron ; 220: 114898, 2023 Jan 15.
Article in English | MEDLINE | ID: covidwho-2120366
ABSTRACT
Analysis of cytokines levels in human serum is critical as it can be a "symptom diagnostic biomarker" in COVID-19, giving real-time information about human health status. Here, we present the construction and performance of a low-price immunosensor (∼US$0.428 per test) based on microfluidic paper-based system to detect cytokine for predicting the health status of COVID-19 patients. Interleukin-6 (IL-6) was selected as the detection model for the close relationship between IL-6 and COVID-19. The assay, which we integrated into foldable paper system, leverages the magnetic immunoassay, the streptavidin-horseradish peroxidase (HRP) associated with tetramethyl benzidine/hydrogen peroxide (TMB/H2O2) to amplify the signal for electrochemical readout. To improve the sensitivity of cytokine detection, a hybrid of gold nanoparticles (AuNPs) and polypyrrole (PPy) hydrogel was modified on the working electrode to increase the conductivity and improve the electron transfer rate. With our prototypic origami paper-based immunosensor operated in differential pulse voltammetry (DPV) mode, we achieved excellent results with a dynamic range from 5 to 1000 pg/mL and a lower detection limit (LOD) of 0.654 pg/mL. Furthermore, we evaluated the capability of the clinical application of the proposed immunosensor using human serum samples from a hospital. The results indicate that our proposed immunosensor has great potential in early diagnosing high-risk COVID-19 patients.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Metal Nanoparticles / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Metal Nanoparticles / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2023 Document Type: Article