Epigenetic drifted UV-light tolerance behavior in multidrug resistant Mycoplasma augmented with biofilm mix recovered from mastitis in Baghdad

ABSTRACT

Biological war or tomorrow war was a forbidden sequel of very dangerous past residues of genes manipulating strategies. Pandemic Corona virus (Covid-19), pandemic Influenza, Hemorrhagic fever, Ebola, AIDS, Methicillin resistant Staphylococcus aureus (MRSA), Extended spectrum beta lactamase resistant Klebsiella pneumonia(ESbetaL), pandemic clones of Acinetobacter baumannii and now forbidden clones of foodborne Mycoplasma series especially M. pneumoniae as a bridge of stress hardening phenomenon associated with Covid 19 within food chain rather than nosocomial infected individuals inside Iraqi ecosystem. Epigenetic temporary drifted tolerance versus genetically permanent shifted resistance behaviors for multidrug antibiotics cascaded by UV radiation abnormal tolerance power was verified in recovered forbidden isolates of biofilm enclosed ecosystems of Mycoplasma species from Cows mastitic milk samples in Baghdad. How can these modified entities survive these struggling, how can learn and adapt to these stressors to be a Chimera through a decade ago and how be transferred from nosocomial environment to community and food chain ecosystem? Uncontrolled genetic engineering power with continuous hidden mutations with forbidden transducing prophages guided by a security chip or trusted platform module of sophisticated brain like machines recognized by quorum sensing mechanisms could be somewhat a partial clue of trueness of deciphered puzzles. Totallya 180 randomly collected pooled raw milk samples from Al-Fudhaliyah, Abu-Ghraib and Al-Sadrya ecosystems throughout March cascaded to July (2021) were verified for abnormal mastitis enrollment cascaded kits. Frequency and distribution patterns for recovered forbidden biofilm clone isolates of Mycoplasma series ecomap were 44 strains (64.7%) out of 68 CMT positive case and totally 180 (24.44%) from which, 20 strains were positive M. pneumoniae (29.41%) and totally (11.11%) verified by a series documented tests of modified methylene blue PPLO agar, biochemical series, scanning electron microscope and PCR technologies. Multidrug resistance behavior was unveiled in 32 strains (72.72%) out of 44 from which, 18 strains were positive M. pneumoniae (40.9%). UV radiation tolerance behavior was encountered in 12 strains (37.5%) out of 32 from which, 9 strains were positive M. pneumoniae (28.125%). In a conclusion remarks, we verified abnormal behavior of UV radiation tolerance in biofilm ecosystem of multidrug resistant strains of Mycoplasma series especially M. pneumoniae recovered from mastitic milk in Baghdad due to genes sharing mechanisms with recovered forbidden clones of Methicillin resistant Staphylococcus aureus (MRSA), Vancomycin resistant Enterococcus faecalis (VRE) and ESbetaL Klebsiella pneumoniae in the same workstation ecosystems. Termination of these new bio life and biohazard emergent infectious foci of abnormality drifted clones of Mycoplasma especially M. pneumoniae needs sophisticated enrollment and association of all sources with Self-denial.