Single-cell RNA-seq methods to interrogate virus-host interactions.
Semin Immunopathol
; 2022 Nov 21.
Article
in English
| MEDLINE | ID: covidwho-2277855
ABSTRACT
The twenty-first century has seen the emergence of many epidemic and pandemic viruses, with the most recent being the SARS-CoV-2-driven COVID-19 pandemic. As obligate intracellular parasites, viruses rely on host cells to replicate and produce progeny, resulting in complex virus and host dynamics during an infection. Single-cell RNA sequencing (scRNA-seq), by enabling broad and simultaneous profiling of both host and virus transcripts, represents a powerful technology to unravel the delicate balance between host and virus. In this review, we summarize technological and methodological advances in scRNA-seq and their applications to antiviral immunity. We highlight key scRNA-seq applications that have enabled the understanding of viral genomic and host response heterogeneity, differential responses of infected versus bystander cells, and intercellular communication networks. We expect further development of scRNA-seq technologies and analytical methods, combined with measurements of additional multi-omic modalities and increased availability of publicly accessible scRNA-seq datasets, to enable a better understanding of viral pathogenesis and enhance the development of antiviral therapeutics strategies.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Language:
English
Journal subject:
Allergy and Immunology
/
Pathology
Year:
2022
Document Type:
Article
Affiliation country:
S00281-022-00972-2
Similar
MEDLINE
...
LILACS
LIS