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Ultrasensitive FRET-based aptasensor for interleukin-6 as a biomarker for COVID-19 progression using nitrogen-doped carbon quantum dots and gold nanoparticles.
Mahani, Mohamad; Faghihi-Fard, Majedeh; Divsar, Faten; Torkzadeh-Mahani, Masoud; Khakbaz, Faeze.
  • Mahani M; Department of Chemistry, Faculty of Chemistry and Chemical Engineering, Graduate University of Advanced Technology, Kerman, 7631818356, Iran. mohmahani@gmail.com.
  • Faghihi-Fard M; Department of Chemistry, Faculty of Chemistry and Chemical Engineering, Graduate University of Advanced Technology, Kerman, 7631818356, Iran.
  • Divsar F; Department of Chemistry, Payame Noor University (PNU), Tehran, 19395-4697, Iran.
  • Torkzadeh-Mahani M; Department of Biotechnology, Institute of Science, High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
  • Khakbaz F; Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran.
Mikrochim Acta ; 189(12): 472, 2022 11 24.
Article in English | MEDLINE | ID: covidwho-2128681
ABSTRACT
A label-free and specific FRET-based interleukin-6 (IL-6) aptasensor was developed using a DNA aptamer modified with nitrogen-doped carbon quantum dots (NCDs) and gold nanoparticles (AuNPs) as a donor-quencher pair. The assayed target was capable of disrupting the donor-acceptor assemblies yielding a concentration-related fluorescence recovery of NCDs (λem = 445 nm and λex = 350 nm). By designing two different probes, the interaction of DNA aptamers with IL-6 protein was studied using FRET efficiency. It appeared that the sensing probes showed slightly different sensing profiles. One of the aptasensors showed a linear response of 1.5-5.9 pg/mL for IL-6 with a coefficient of determination of R2 ≥ 0.99 and the a detection limit of 0.82 pg/mL (at S/N = 3). The experimental results indicated that the biosensor can be applied to determine IL-6 in human serum (with recovery of 95.7-102.9%). Due to the high sensitivity, excellent selectivity, and simplicity of the procedure, this strategy represents a promising alternative for IL-6 sensing in clinical applications.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Quantum Dots / Aptamers, Nucleotide / Metal Nanoparticles / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Mikrochim Acta Year: 2022 Document Type: Article Affiliation country: S00604-022-05570-5

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Quantum Dots / Aptamers, Nucleotide / Metal Nanoparticles / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Mikrochim Acta Year: 2022 Document Type: Article Affiliation country: S00604-022-05570-5