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Critical Offset Magnetic PArticle SpectroScopy for rapid and highly sensitive medical point-of-care diagnostics.
Vogel, Patrick; Rückert, Martin Andreas; Friedrich, Bernhard; Tietze, Rainer; Lyer, Stefan; Kampf, Thomas; Hennig, Thomas; Dölken, Lars; Alexiou, Christoph; Behr, Volker Christian.
  • Vogel P; Department of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Würzburg, Germany. Patrick.Vogel@physik.uni-wuerzburg.de.
  • Rückert MA; Department of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Würzburg, Germany.
  • Friedrich B; Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, University Hospital Erlangen, Erlangen, Germany.
  • Tietze R; Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, University Hospital Erlangen, Erlangen, Germany.
  • Lyer S; Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Professorship for AI-Controlled Nanomaterials, University Hospital Erlangen, Erlangen, Germany.
  • Kampf T; Department of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Würzburg, Germany.
  • Hennig T; Department of Diagnostic and Interventional Neuroradiology, University Hospital Würzburg, Würzburg, Germany.
  • Dölken L; Institute for Virology and Immunobiology, Julius-Maximilians-University Würzburg, Würzburg, Germany.
  • Alexiou C; Institute for Virology and Immunobiology, Julius-Maximilians-University Würzburg, Würzburg, Germany.
  • Behr VC; Helmholtz Institute for RNA-based Infection Research, Helmholtz-Center for Infection Research, Würzburg, Germany.
Nat Commun ; 13(1): 7230, 2022 Nov 24.
Article in English | MEDLINE | ID: covidwho-2133434
ABSTRACT
Magnetic nanoparticles (MNPs) have been adapted for many applications, e.g., bioassays for the detection of biomarkers such as antibodies, by controlled engineering of specific surface properties. Specific measurement of such binding states is of high interest but currently limited to highly sensitive techniques such as ELISA or flow cytometry, which are relatively inflexible, difficult to handle, expensive and time-consuming. Here we report a method named COMPASS (Critical-Offset-Magnetic-Particle-SpectroScopy), which is based on a critical offset magnetic field, enabling sensitive detection to minimal changes in mobility of MNP ensembles, e.g., resulting from SARS-CoV-2 antibodies binding to the S antigen on the surface of functionalized MNPs. With a sensitivity of 0.33 fmole/50 µl (≙7 pM) for SARS-CoV-2-S1 antibodies, measured with a low-cost portable COMPASS device, the proposed technique is competitive with respect to sensitivity while providing flexibility, robustness, and a measurement time of seconds per sample. In addition, initial results with blood serum demonstrate high specificity.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Magnetite Nanoparticles / COVID-19 Type of study: Diagnostic study / Experimental Studies Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2022 Document Type: Article Affiliation country: S41467-022-34941-y

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Magnetite Nanoparticles / COVID-19 Type of study: Diagnostic study / Experimental Studies Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2022 Document Type: Article Affiliation country: S41467-022-34941-y