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Plasma metabolomics and gene regulatory networks analysis reveal the role of nonstructural SARS-CoV-2 viral proteins in metabolic dysregulation in COVID-19 patients.
Ivanisenko, V A; Gaisler, E V; Basov, N V; Rogachev, A D; Cheresiz, S V; Ivanisenko, T V; Demenkov, P S; Mishchenko, E L; Khripko, O P; Khripko, Yu I; Voevoda, S M; Karpenko, T N; Velichko, A J; Voevoda, M I; Kolchanov, N A; Pokrovsky, A G.
  • Ivanisenko VA; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia. salix@bionet.nsc.ru.
  • Gaisler EV; Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia. salix@bionet.nsc.ru.
  • Basov NV; Kurchatov Genomics Center of Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia. salix@bionet.nsc.ru.
  • Rogachev AD; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.
  • Cheresiz SV; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.
  • Ivanisenko TV; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.
  • Demenkov PS; N. N. Vorozhtsov, Novosibirsk Institute of Organic Chemistry, Acad. Lavrentiev Ave., 9, 630090, Novosibirsk, Russia.
  • Mishchenko EL; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.
  • Khripko OP; Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia.
  • Khripko YI; Kurchatov Genomics Center of Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia.
  • Voevoda SM; Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia.
  • Karpenko TN; Kurchatov Genomics Center of Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia.
  • Velichko AJ; Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia.
  • Voevoda MI; Federal Research Center for Fundamental and Translational Medicine, Timakov Str., 2, 630117, Novosibirsk, Russia.
  • Kolchanov NA; Federal Research Center for Fundamental and Translational Medicine, Timakov Str., 2, 630117, Novosibirsk, Russia.
  • Pokrovsky AG; Federal Research Center for Fundamental and Translational Medicine, Timakov Str., 2, 630117, Novosibirsk, Russia.
Sci Rep ; 12(1): 19977, 2022 Nov 20.
Article in English | MEDLINE | ID: covidwho-2133615
ABSTRACT
Metabolomic analysis of blood plasma samples from COVID-19 patients is a promising approach allowing for the evaluation of disease progression. We performed the metabolomic analysis of plasma samples of 30 COVID-19 patients and the 19 controls using the high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometric detection (LC-MS/MS). In our analysis, we identified 103 metabolites enriched in KEGG metabolic pathways such as amino acid metabolism and the biosynthesis of aminoacyl-tRNAs, which differed significantly between the COVID-19 patients and the controls. Using ANDSystem software, we performed the reconstruction of gene networks describing the potential genetic regulation of metabolic pathways perturbed in COVID-19 patients by SARS-CoV-2 proteins. The nonstructural proteins of SARS-CoV-2 (orf8 and nsp5) and structural protein E were involved in the greater number of regulatory pathways. The reconstructed gene networks suggest the hypotheses on the molecular mechanisms of virus-host interactions in COVID-19 pathology and provide a basis for the further experimental and computer studies of the regulation of metabolic pathways by SARS-CoV-2 proteins. Our metabolomic analysis suggests the need for nonstructural protein-based vaccines and the control strategy to reduce the disease progression of COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Experimental Studies Topics: Vaccines Limits: Humans Language: English Journal: Sci Rep Year: 2022 Document Type: Article Affiliation country: S41598-022-24170-0

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Experimental Studies Topics: Vaccines Limits: Humans Language: English Journal: Sci Rep Year: 2022 Document Type: Article Affiliation country: S41598-022-24170-0