Identification and analyses of the proteins interacting with the nucleocapsid protein of the Porcine Deltacoronavirus
Chinese Journal of Virology
; 37(4):900-909, 2021.
Article
in Chinese
| CAB Abstracts | ID: covidwho-2145388
ABSTRACT
Preliminary screening and identification of the host proteins interacting with the nucleocapsid(N)protein of the porcine deltacoronavirus(DCoV). Co-immunoprecipitation and liquid chromatography- tandem mass spectrometry were used to screen out the host proteins interacting with the N protein of the porcine DCoV. Bioinformatics analysis was carried out, and then co-immunoprecipitation was used for identification. Sodium dodecyl sulfate - polyacrylamide gel electrophoresis of the immunoprecipitation products revealed different protein bands around 40 kDa and 100 kDa. Sixty-eight host proteins interacting with the N protein of the porcine DCoV were screened by mass spectrometry. Two candidate interacting proteins(ANXA2 and TUBB2 B)were selected by analyses using the Gene Ontology, Clusters of Orthologous Groups, and Kyoto Encyclopedia of Genes and Genomes databases. After co-immunoprecipitation verification, the N protein of the porcine DCoV was found to interact with TUBB2 B. Our study provides a new direction for further exploration of the role of the N protein of the porcine DCoV in infection.
Full text:
Available
Collection:
Databases of international organizations
Database:
CAB Abstracts
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2021
Document Type:
Article
Similar
MEDLINE
...
LILACS
LIS