Characterizing viral samples using machine learning for Raman and absorption spectroscopy.
Microbiologyopen
; 11(6): e1336, 2022 12.
Article
in English
| MEDLINE | ID: covidwho-2148408
ABSTRACT
Machine learning methods can be used as robust techniques to provide invaluable information for analyzing biological samples in pharmaceutical industries, such as predicting the concentration of viral particles of interest in biological samples. Here, we utilized both convolutional neural networks (CNNs) and random forests (RFs) to predict the concentration of the samples containing measles, mumps, rubella, and varicella-zoster viruses (ProQuad®) based on Raman and absorption spectroscopy. We prepared Raman and absorption spectra data sets with known concentration values, then used the Raman and absorption signals individually and together to train RFs and CNNs. We demonstrated that both RFs and CNNs can make predictions with R2 values as high as 95%. We proposed two different networks to jointly use the Raman and absorption spectra, where our results demonstrated that concatenating the Raman and absorption data increases the prediction accuracy compared to using either Raman or absorption spectrum alone. Additionally, we further verified the advantage of using joint Raman-absorption with principal component analysis. Furthermore, our method can be extended to characterize properties other than concentration, such as the type of viral particles.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Machine Learning
Type of study:
Prognostic study
/
Randomized controlled trials
Language:
English
Journal:
Microbiologyopen
Year:
2022
Document Type:
Article
Affiliation country:
Mbo3.1336
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