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Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein.
Drobysh, Maryia; Ramanavicius, Arunas; Baradoke, Ausra.
  • Drobysh M; State Research Institute Center for Physical and Technological Sciences, Sauletekio ave. 3, Vilnius, Lithuania; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, 03225 Vilnius, Lithuania.
  • Ramanavicius A; State Research Institute Center for Physical and Technological Sciences, Sauletekio ave. 3, Vilnius, Lithuania; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, 03225 Vilnius, Lithuania. Electronic address: Arunas.Ramanavicius@chf.vu.lt.
  • Baradoke A; State Research Institute Center for Physical and Technological Sciences, Sauletekio ave. 3, Vilnius, Lithuania.
Sci Total Environ ; 862: 160700, 2023 Mar 01.
Article in English | MEDLINE | ID: covidwho-2150569
ABSTRACT
In this work, we report an impedimetric system for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein. The sensing platform is based on recombinant Spike protein (SCoV2-rS) immobilized on the phytic acid doped polyaniline films (PANI-PA). The affinity interaction between immobilized SCoV2-rS protein and antibodies in the physiological range of concentrations was registered by electrochemical impedance spectroscopy. Analytical parameters of the sensing platform were tuned by the variation of electropolymerization times during the synthesis of PANI-PA films. The lowest limit of detection and quantification were obtained for electropolymerization time of 20 min and equalled 8.00 ± 0.20 nM and 23.93 ± 0.60 nM with an equilibrium dissociation constant of 3 nM. The presented sensing system is label-free and suitable for the direct detection of antibodies against SARS-CoV-2 in real patient serum samples after coronavirus disease 2019 and/or vaccination.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Topics: Vaccines Limits: Humans Language: English Journal: Sci Total Environ Year: 2023 Document Type: Article Affiliation country: J.scitotenv.2022.160700

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Topics: Vaccines Limits: Humans Language: English Journal: Sci Total Environ Year: 2023 Document Type: Article Affiliation country: J.scitotenv.2022.160700