Visual and Ultrasensitive Detection of a Coronavirus Using a Gold Nanorod Probe under Dark Field.
Biosensors (Basel)
; 12(12)2022 Dec 08.
Article
in English
| MEDLINE | ID: covidwho-2154896
ABSTRACT
Porcine epidemic diarrhea virus (PEDV), a coronavirus that causes highly infectious intestinal diarrhea in piglets, has led to severe economic losses worldwide. Rapid diagnosis and timely supervision are significant in the prophylaxis of PEDV. Herein, we proposed a gold-nanorod (GNR) probe-assisted counting method using dark field microscopy (DFM). The antibody-functionalized silicon chips were prepared to capture PEDV to form sandwich structures with GNR probes for imaging under DFM. Results show that our DFM-based assay for PEDV has a sensitivity of 23.80 copies/µL for simulated real samples, which is very close to that of qPCR in this study. This method of GNR probes combined with DFM for quantitative detection of PEDV not only has strong specificity, good repeatability, and a low detection limit, but it also can be implemented for rapid on-site detection of the pathogens.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Swine Diseases
/
Coronavirus Infections
/
Coronavirus
/
Porcine epidemic diarrhea virus
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Year:
2022
Document Type:
Article
Affiliation country:
Bios12121146
Similar
MEDLINE
...
LILACS
LIS