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SARS-CoV-2 multi-variant rapid detector based on graphene transistor functionalized with an engineered dimeric ACE2 receptor.
Romagnoli, Alice; D'Agostino, Mattia; Pavoni, Eleonora; Ardiccioni, Chiara; Motta, Stefano; Crippa, Paolo; Biagetti, Giorgio; Notarstefano, Valentina; Rexha, Jesmina; Perta, Nunzio; Barocci, Simone; Costabile, Brianna K; Colasurdo, Gabriele; Caucci, Sara; Mencarelli, Davide; Turchetti, Claudio; Farina, Marco; Pierantoni, Luca; La Teana, Anna; Al Hadi, Richard; Cicconardi, Francesco; Chinappi, Mauro; Trucchi, Emiliano; Mancia, Filippo; Menzo, Stefano; Morozzo Della Rocca, Blasco; D'Annessa, Ilda; Di Marino, Daniele.
  • Romagnoli A; Department of Life and Environmental Sciences, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • D'Agostino M; National Biodiversity Future Center (NBFC), Palermo, Italy.
  • Pavoni E; New York-Marche Structural Biology Center (NY-MaSBiC), Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Ardiccioni C; Department of Life and Environmental Sciences, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Motta S; Department of Information Engineering, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Crippa P; Department of Life and Environmental Sciences, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Biagetti G; New York-Marche Structural Biology Center (NY-MaSBiC), Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Notarstefano V; Department of Earth and Environmental Sciences, University of Milano-Bicocca, Milan, Italy.
  • Rexha J; Department of Information Engineering, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Perta N; Department of Information Engineering, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Barocci S; Department of Life and Environmental Sciences, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Costabile BK; Department of Life and Environmental Sciences, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Colasurdo G; National Biodiversity Future Center (NBFC), Palermo, Italy.
  • Caucci S; Department of Life and Environmental Sciences, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Mencarelli D; National Biodiversity Future Center (NBFC), Palermo, Italy.
  • Turchetti C; Department of Clinical Pathology, ASUR Marche AV1, Urbino, PU, Italy.
  • Farina M; Department of Physiology and Cellular Biophysics, Columbia University, New York, NY 10032, USA.
  • Pierantoni L; Department of Architecture, Bologna University, Bologna 40126, Italy.
  • La Teana A; Virology Unit, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Torrette, 60126 Ancona, Italy.
  • Al Hadi R; Department of Information Engineering, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Cicconardi F; Department of Information Engineering, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Chinappi M; Department of Information Engineering, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Trucchi E; Department of Information Engineering, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Mancia F; Department of Life and Environmental Sciences, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • Menzo S; National Biodiversity Future Center (NBFC), Palermo, Italy.
  • Morozzo Della Rocca B; New York-Marche Structural Biology Center (NY-MaSBiC), Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy.
  • D'Annessa I; Alcatera Inc., 1401 Westwood Blvd Suite 280, Los Angeles, CA 90024, USA.
  • Di Marino D; School of Biological Sciences, University of Bristol, Life Sciences Building, 24 Tyndall Ave, Bristol BS8 1TQ, UK.
Nano Today ; 48: 101729, 2023 Feb.
Article in English | MEDLINE | ID: covidwho-2159612
ABSTRACT
Reliable point-of-care (POC) rapid tests are crucial to detect infection and contain the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The emergence of several variants of concern (VOC) can reduce binding affinity to diagnostic antibodies, limiting the efficacy of the currently adopted tests, while showing unaltered or increased affinity for the host receptor, angiotensin converting enzyme 2 (ACE2). We present a graphene field-effect transistor (gFET) biosensor design, which exploits the Spike-ACE2 interaction, the crucial step for SARS-CoV-2 infection. Extensive computational analyses show that a chimeric ACE2-Fragment crystallizable (ACE2-Fc) construct mimics the native receptor dimeric conformation. ACE2-Fc functionalized gFET allows in vitro detection of the trimeric Spike protein, outperforming functionalization with a diagnostic antibody or with the soluble ACE2 portion, resulting in a sensitivity of 20 pg/mL. Our miniaturized POC biosensor successfully detects B.1.610 (pre-VOC), Alpha, Beta, Gamma, Delta, Omicron (i.e., BA.1, BA.2, BA.4, BA.5, BA.2.75 and BQ.1) variants in isolated viruses and patient's clinical nasopharyngeal swabs. The biosensor reached a Limit Of Detection (LOD) of 65 cps/mL in swab specimens of Omicron BA.5. Our approach paves the way for a new and reusable class of highly sensitive, rapid and variant-robust SARS-CoV-2 detection systems.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Prognostic study Topics: Variants Language: English Journal: Nano Today Year: 2023 Document Type: Article Affiliation country: J.nantod.2022.101729

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Prognostic study Topics: Variants Language: English Journal: Nano Today Year: 2023 Document Type: Article Affiliation country: J.nantod.2022.101729