Lab-on-a-chip for the easy and visual detection of SARS-CoV-2 in saliva based on sensory polymers.
Sens Actuators B Chem
; 379: 133165, 2023 Mar 15.
Article
in English
| MEDLINE | ID: covidwho-2159830
ABSTRACT
The initial stages of the pandemic caused by SARS-CoV-2 showed that early detection of the virus in a simple way is the best tool until the development of vaccines. Many different tests are invasive or need the patient to cough up or even drag a sample of mucus from the throat area. Besides, the manufacturing time has proven insufficient in pandemic conditions since they were out of stock in many countries. Here we show a new method of manufacturing virus sensors and a proof of concept with SARS-CoV-2. We found that a fluorogenic peptide substrate of the main protease of the virus (Mpro) can be covalently immobilized in a polymer, with which a cellulose-based material can be coated. These sensory labels fluoresce with a single saliva sample of a positive COVID-19 patient. The results matched with that of the antigen tests in 22 of 26 studied cases (85% success rate).
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Type of study:
Diagnostic study
/
Screening_studies
Topics:
Vaccines
Language:
English
Journal:
Sens Actuators B Chem
Year:
2023
Document Type:
Article
Affiliation country:
J.snb.2022.133165
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