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An Electrochemical Immunosensor Based on Carboxylated Graphene/SPCE for IgG-SARS-CoV-2 Nucleocapsid Determination.
de Souza Freire, Luciana; Ruzo, Camila Macena; Salgado, Bárbara Batista; Gandarilla, Ariamna María Dip; Romaguera-Barcelay, Yonny; Tavares, Ana P M; Sales, Maria Goreti Ferreira; Cordeiro, Isabelle; Lalwani, Jaila Dias Borges; Matos, Robert; Fonseca Filho, Henrique; Astolfi-Filho, Spartaco; Talu, Stefan; Lalwani, Pritesh; Brito, Walter Ricardo.
  • de Souza Freire L; Department of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Manaus 69067-005, AM, Brazil.
  • Ruzo CM; Department of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Manaus 69067-005, AM, Brazil.
  • Salgado BB; Instituto Leônidas e Maria Deane (ILMD), Fiocruz Amazônia, Manaus 69067-005, AM, Brazil.
  • Gandarilla AMD; Department of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Manaus 69067-005, AM, Brazil.
  • Romaguera-Barcelay Y; Department of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Manaus 69067-005, AM, Brazil.
  • Tavares APM; BioMark@UC, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, 3030-790 Coimbra, Portugal.
  • Sales MGF; BioMark@UC, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, 3030-790 Coimbra, Portugal.
  • Cordeiro I; BioMark@UC, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, 3030-790 Coimbra, Portugal.
  • Lalwani JDB; Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Amazonas, Manaus 69067-005, AM, Brazil.
  • Matos R; Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus 69067-005, AM, Brazil.
  • Fonseca Filho H; Amazonian Materials Group, Federal University of Amapá (UNIFAP), Macapá 49100-000, AP, Brazil.
  • Astolfi-Filho S; Laboratory of Nanomaterials Synthesis and Nanoscopy (LSNN), Federal University of Amazonas (UFAM), Manaus 69067-005, AM, Brazil.
  • Talu S; Department of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Manaus 69067-005, AM, Brazil.
  • Lalwani P; PPGBIOTEC, Federal University of Amazonas, Manaus 69067-005, AM, Brazil.
  • Brito WR; The Directorate of Research, Development and Innovation Management (DMCDI), The Technical University of Cluj-Napoca, Constantin Daicoviciu Street, No. 15, 400020 Cluj-Napoca, Romania.
Biosensors (Basel) ; 12(12)2022 Dec 13.
Article in English | MEDLINE | ID: covidwho-2163244
ABSTRACT
The COVID-19 pandemic has emphasized the importance and urgent need for rapid and accurate diagnostic tests for detecting and screening this infection. Our proposal was to develop a biosensor based on an ELISA immunoassay for monitoring antibodies against SARS-CoV-2 in human serum samples. The nucleocapsid protein (N protein) from SARS-CoV-2 was employed as a specific receptor for the detection of SARS-CoV-2 nucleocapsid immunoglobulin G. N protein was immobilized on the surface of a screen-printed carbon electrode (SPCE) modified with carboxylated graphene (CG). The percentage of IgG-SARS-CoV-2 nucleocapsid present was quantified using a secondary antibody labeled with horseradish peroxidase (HRP) (anti-IgG-HRP) catalyzed using 3,3',5,5'-tetramethylbenzidine (TMB) mediator by chronoamperometry. A linear response was obtained in the range of 11000-1200 v/v in phosphate buffer solution (PBS), and the detection limit calculated was 14947 v/v. The chronoamperometric method showed electrical signals directly proportional to antibody concentrations due to antigen-antibody (Ag-Ab) specific and stable binding reaction.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 / Graphite Type of study: Diagnostic study Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Bios12121161

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 / Graphite Type of study: Diagnostic study Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Bios12121161