Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing.
Medicina (Kaunas)
; 58(12)2022 Dec 15.
Article
in English
| MEDLINE | ID: covidwho-2163508
ABSTRACT
Background and Objectives:
SARS-CoV-2 is the first global threat and life-changing event of the twenty-first century. Although efficient treatments and vaccines have been developed, due to the virus's ability to mutate in key regions of the genome, whole viral genome sequencing is needed for efficient monitoring, evaluation of the spread, and even the adjustment of the molecular diagnostic assays. Materials andMethods:
In this study, Nanopore and Ion Torrent sequencing technologies were used to detect the main SARS-CoV-2 circulating strains in Timis County, Romania, between February 2021 and May 2022.Results:
We identified 22 virus lineages belonging to seven clades 20A, 20I (Alpha, V1), 21B (Kappa), 21I (Delta), 21J (Delta), 21K (Omicron), and 21L (Omicron).Conclusions:
Results obtained with both methods are comparable, and we confirm the utility of Nanopore sequencing in large-scale epidemiological surveillance due to the lower cost and reduced time for library preparation.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Nanopore Sequencing
/
COVID-19
Type of study:
Diagnostic study
/
Experimental Studies
/
Observational study
Topics:
Vaccines
/
Variants
Limits:
Humans
Language:
English
Journal subject:
Medicine
Year:
2022
Document Type:
Article
Affiliation country:
Medicina58121841
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