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Rapid Quantification of SARS-CoV-2 Neutralising Antibodies Using Time-Resolved Fluorescence Immunoassay.
McLean, Gary R; Zhang, Yueke; Ndoyi, Rene; Martin, Adam; Winer, Julian.
  • McLean GR; School of Human Sciences, London Metropolitan University, London N7 8DB, UK.
  • Zhang Y; National Heart and Lung Institute, Imperial College London, London W2 1PG, UK.
  • Ndoyi R; PremaLabs Diagnostics UK Ltd., London W1J 6ER, UK.
  • Martin A; PremaLabs Diagnostics UK Ltd., London W1J 6ER, UK.
  • Winer J; PremaLabs Diagnostics UK Ltd., London W1J 6ER, UK.
Vaccines (Basel) ; 10(12)2022 Dec 15.
Article in English | MEDLINE | ID: covidwho-2163726
ABSTRACT
The quantification of neutralising antibodies (NAb) for SARS-CoV-2 has become an important tool for monitoring protective immunity following infection or immunisation. In this study, we evaluated using World-Health-Organisation-standard immunoglobulin preparations, a novel point-of-care test that quantitates NAb by time-resolved fluorescent immunoassay. The assay provided robust data of binding antibody units (BAU) in 15 min that were well correlated with NAb values obtained by traditional in vitro neutralisation assay. The data also correlated well to spike-receptor-binding domain-binding antibodies over a broad range of plasma dilutions. The assay was extremely sensitive, able to detect positive samples after dilution 110,000 and over a wide range of BAU. Assay specificity was estimated at 96% using Pre-COVID-19 serum samples when applying a cut-off value of 47 BAU/mL, although readings of up to 100 BAU/mL could be considered borderline. This point-of-care diagnostic test is useful for rapid population screening and includes the use of capillary blood samples. Furthermore, it provides results for SARS-CoV-2 NAb in 15 min, which can inform immediate decisions regarding protective immunity levels and the need for continued COVID immunisations.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Experimental Studies Language: English Year: 2022 Document Type: Article Affiliation country: Vaccines10122149

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Experimental Studies Language: English Year: 2022 Document Type: Article Affiliation country: Vaccines10122149