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A novel paper-based lysis strip for SARS-CoV-2 RNA detection at low resource settings.
Lee, Songhyun; Kim, SeJin; Kim, Sanghyo.
  • Lee S; Department of Bionanotechnology, Gachon University, Seongnam, 13120, Republic of Korea.
  • Kim S; Department of Bionanotechnology, Gachon University, Seongnam, 13120, Republic of Korea.
  • Kim S; Department of Bionanotechnology, Gachon University, Seongnam, 13120, Republic of Korea. Electronic address: samkim@gachon.ac.kr.
Anal Biochem ; 664: 115037, 2023 03 01.
Article in English | MEDLINE | ID: covidwho-2164921
ABSTRACT
Infectious respiratory diseases such as COVID-19 are serious and global concerns from the past to the present. To isolate the spread of infectious diseases even in the absence of a health system, a simple, inexpensive, reliable, sensitive, and selective molecular diagnosis platform for Point of Care Test (POCT) is required. Especially, the nucleic acid extraction step is difficult to perform out of laboratory. Here, we propose a paper-based lysis (PBL) strip for nucleic acid extraction, especially in low-resource settings (LRS). PBL strips are suitable for isolating RNA from viruses with biological interference and inhibitors. We optimized the buffer compositions and membranes of the strip. A simple preparation method using a PBL strip could obtain an eluent for downstream inspection within 20 min. Overall, 104 copies/swaps were detected for 20 min for amplification in combination with Reverse Transcription Loop-Mediated Amplification (RT-LAMP).
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acids / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Anal Biochem Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acids / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Anal Biochem Year: 2023 Document Type: Article