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A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus.
Robin, Perrine; Barnabei, Laura; Marocco, Stefano; Pagnoncelli, Jacopo; Nicolis, Daniele; Tarantelli, Chiara; Tavilla, Agatino Christian; Robortella, Roberto; Cascione, Luciano; Mayoraz, Lucas; Journot, Céline M A; Mensi, Mounir; Bertoni, Francesco; Stefanini, Igor; Gerber-Lemaire, Sandrine.
  • Robin P; Group for Functionalized Biomaterials, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
  • Barnabei L; Institute of Oncology Research, Faculty of Biomedical Sciences, USI, Via Francesco Chiesa 5, CH-6500, Bellinzona, Switzerland.
  • Marocco S; Medical Devices area, Institute of Digital Technologies for Personalized Healthcare - MeDiTech, Department of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, Switzerland.
  • Pagnoncelli J; Medical Devices area, Institute of Digital Technologies for Personalized Healthcare - MeDiTech, Department of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, Switzerland.
  • Nicolis D; Department of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, Switzerland.
  • Tarantelli C; Institute of Oncology Research, Faculty of Biomedical Sciences, USI, Via Francesco Chiesa 5, CH-6500, Bellinzona, Switzerland.
  • Tavilla AC; Department of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, Switzerland.
  • Robortella R; Department of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, Switzerland.
  • Cascione L; Institute of Oncology Research, Faculty of Biomedical Sciences, USI, Via Francesco Chiesa 5, CH-6500, Bellinzona, Switzerland.
  • Mayoraz L; Group for Functionalized Biomaterials, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
  • Journot CMA; Group for Functionalized Biomaterials, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
  • Mensi M; ISIC-XRDSAP, EPFL Valais-Wallis, Rue de l'Industrie 17, CH-1951, Sion, Switzerland.
  • Bertoni F; Institute of Oncology Research, Faculty of Biomedical Sciences, USI, Via Francesco Chiesa 5, CH-6500, Bellinzona, Switzerland.
  • Stefanini I; Oncology Institute of Southern Switzerland, Ente Ospedaliero Cantonale, CH-6500, Bellinzona, Switzerland.
  • Gerber-Lemaire S; Medical Devices area, Institute of Digital Technologies for Personalized Healthcare - MeDiTech, Department of Innovative Technologies, University of Applied Sciences of Southern Switzerland, Via la Santa 1, CH-6962, Lugano, Viganello, Switzerland.
Biosens Bioelectron X ; 13: 100302, 2023 May.
Article in English | MEDLINE | ID: covidwho-2165112
ABSTRACT
The emergence of the coronavirus 2019 (COVID-19) arose the need for rapid, accurate and massive virus detection methods to control the spread of infectious diseases. In this work, a device, deployable in non-medical environments, has been developed for the detection of non-amplified SARS-CoV-2 RNA. A SARS-CoV-2 specific probe was designed and covalently immobilized at the surface of glass slides to fabricate a DNA biosensor. The resulting system was integrated in a microfluidic platform, in which viral RNA was extracted from non-treated human saliva, before hybridizing at the surface of the sensor. The formed DNA/RNA duplex was detected in presence of SYBR Green I using an opto-electronic system, based on a high-power LED and a photo multiplier tube, which convert the emitted fluorescence into an electrical signal that can be processed in less than 10 min. The limit of detection of the resulting microfluidic platform reached six copies of viral RNA per microliter of sample (equal to 10 aM) and satisfied the safety margin. The absence of non-specific adsorption and the selectivity for SARS-CoV-2 RNA were established. In addition, the designed device could be applicable for the detection of a variety of viruses by simple modification of the immobilized probe.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Language: English Journal: Biosens Bioelectron X Year: 2023 Document Type: Article Affiliation country: J.biosx.2022.100302

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Language: English Journal: Biosens Bioelectron X Year: 2023 Document Type: Article Affiliation country: J.biosx.2022.100302