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Mechanical tuning of virus-like particles.
Radiom, Milad; Keys, Tim; Turgay, Yagmur; Ali, Ahmed; Preet, Swapan; Chesnov, Serge; Lutz-Bueno, Viviane; Slack, Emma; Mezzenga, Raffaele.
  • Radiom M; Laboratory of Food Immunology, Institute of Food, Nutrition and Health, ETH Zürich, Zürich, Switzerland; Laboratory of Food and Soft Materials, Institute of Food, Nutrition and Health, ETH Zürich, Zürich, Switzerland. Electronic address: milad.radiom@hest.ethz.ch.
  • Keys T; Laboratory of Food Immunology, Institute of Food, Nutrition and Health, ETH Zürich, Zürich, Switzerland.
  • Turgay Y; Laboratory of Food Immunology, Institute of Food, Nutrition and Health, ETH Zürich, Zürich, Switzerland.
  • Ali A; Laboratory of Food Immunology, Institute of Food, Nutrition and Health, ETH Zürich, Zürich, Switzerland.
  • Preet S; Laboratory of Food Immunology, Institute of Food, Nutrition and Health, ETH Zürich, Zürich, Switzerland.
  • Chesnov S; University of Zürich/ETH Zürich, Functional Genomics Centre Zürich, Zürich, Switzerland.
  • Lutz-Bueno V; Paul Scherrer Institute PSI, Villigen, Switzerland.
  • Slack E; Laboratory of Food Immunology, Institute of Food, Nutrition and Health, ETH Zürich, Zürich, Switzerland. Electronic address: emma.slack@hest.ethz.ch.
  • Mezzenga R; Laboratory of Food and Soft Materials, Institute of Food, Nutrition and Health, ETH Zürich, Zürich, Switzerland. Electronic address: raffaele.mezzenga@hest.ethz.ch.
J Colloid Interface Sci ; 634: 963-971, 2023 Mar 15.
Article in English | MEDLINE | ID: covidwho-2244438
ABSTRACT

HYPOTHESIS:

Virus-like particles (VLPs) are promising scaffolds for developing mucosal vaccines. For their optimal performance, in addition to design parameters from an immunological perspective, biophysical properties may need to be considered. EXPERIMENTS We investigated the mechanical properties of VLPs scaffolded on the coat protein of Acinetobacter phage AP205 using atomic force microscopy and small angle X-ray scattering.

FINDINGS:

Investigations showed that AP205 VLP is a tough nanoshell of stiffness 93 ± 23 pN/nm and elastic modulus 0.11 GPa. However, its mechanical properties are modulated by attaching muco-inert polyethylene glycol to 46 ± 10 pN/nm and 0.05 GPa. Addition of antigenic peptides derived from SARS-CoV2 spike protein by genetic fusion increased the stiffness to 146 ± 54 pN/nm although the elastic modulus remained unchanged. These results, which are interpreted in terms of shell thickness and coat protein net charge variations, demonstrate that surface conjugation can induce appreciable changes in the biophysical properties of VLP-scaffolded vaccines.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bacteriophages / Vaccines, Virus-Like Particle / COVID-19 Topics: Vaccines Limits: Humans Language: English Journal: J Colloid Interface Sci Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bacteriophages / Vaccines, Virus-Like Particle / COVID-19 Topics: Vaccines Limits: Humans Language: English Journal: J Colloid Interface Sci Year: 2023 Document Type: Article