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Detection of SARS-CoV-2 neutralizing antibodies: reliability of serological tests
Biochimica Clinica ; 46(3):S170, 2022.
Article in English | EMBASE | ID: covidwho-2168852
ABSTRACT
Serological assays allow to explore the synthesis of antibodies against SARS-CoV-2 proteins. The gained knowledge finds applications in sero-epidemiology studies, to develop accurate forecasts of protective immunity spreading within a population. Indeed, last February, the European Centre for Disease prevention and Control (ECDC) recommended the use of serological tests for population surveys, in the context of epidemiological studies. The up-to-date reference method to determine the amount of protective antibodies is the in vitro neutralization test, which is expensive, timeconsuming and requires biosafety level 3 laboratories. The current study aims at highlighting the correlation between the results of the reference method and four different serological assays. Three immunoassays (Elecsys Anti SARS-CoV-2 S, MAGLUMI SARSCoV- 2 S-RBD IgG and MAGLUMI 2019-nCoV IgG test) and an ELISA surrogate viral neutralization test (EUROIMMUN SARS-CoV-2 NeutraLISA assay) have been studied on 83 patients, previously infected by SARS-CoV-2. The correlation between each serological test and the reference method is reported, as well as the predictive performances to distinguish serum samples with neutralizing antibody titers higher than 160. All the assays achieved good performances in terms of correlation with the reference method, as well as concerning the predictive ability over NAb titers > 160. The best correlation (Spearman coefficient = 0.784) and predictive features (area under curve = 0.921) have been observed for EUROIMMUN SARS-CoV-2 NeutraLISA assay, as displayed by ROC curve analyses.
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Collection: Databases of international organizations Database: EMBASE Language: English Journal: Biochimica Clinica Year: 2022 Document Type: Article

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Collection: Databases of international organizations Database: EMBASE Language: English Journal: Biochimica Clinica Year: 2022 Document Type: Article