Real-Time RT-PCR Detection of Equine Coronavirus

Miszczak, F.; Kin, N.; Tesson, V.; Vabret, A.

Miszczak, F.; Kin, N.; Tesson, V.; Vabret, A..

Springer Protocols Handbooks ; : 93-100, 2022.

Article in English | EMBASE | ID: covidwho-2173505

ABSTRACT

ABSTRACT

Equine coronavirus (ECoV) is a recently identified equine virus, involved mainly in enteric infections. Since the ECoV discovery in 1999, only two real-time RT-PCRs have been developed for viral identification. In this chapter we describe a one-step real-time RT-PCR that has been routinely used in our laboratory for ECoV detection from fecal and respiratory samples. Copyright © Springer Science+Business Media New York 2016.

Keywords

Clinical diagnosis; ECoV; Molecular detection; Real-time RT-PCR; Coronavirinae; Equus; feces; nonhuman; real time reverse transcription polymerase chain reaction

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Full text: Available Collection: Databases of international organizations Database: EMBASE Language: English Journal: Springer Protocols Handbooks Year: 2022 Document Type: Article

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