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PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene
Springer Protocols Handbooks ; : 151-160, 2022.
Article in English | EMBASE | ID: covidwho-2173510
ABSTRACT
Turkey coronaviral enteritis caused by turkey coronavirus (TCoV) continues to infect turkey flocks, resulting in significant economic loss. Determining and understanding genetic relationships among different TCoV isolates or strains is important for controlling the disease. Using two-step RT-PCR assays that amplify the full length of TCoV spike (S) gene, TCoV isolates can be sequenced, analyzed, and genotyped. Described in this chapter is the protocol on PCR amplification and sequencing analysis of full-length TCoV S gene. Such protocol is useful in molecular epidemiology for establishing an effective strategy to control the transmission of TCoV among turkey flocks. Copyright © Springer Science+Business Media New York 2016
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Full text: Available Collection: Databases of international organizations Database: EMBASE Language: English Journal: Springer Protocols Handbooks Year: 2022 Document Type: Article

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Full text: Available Collection: Databases of international organizations Database: EMBASE Language: English Journal: Springer Protocols Handbooks Year: 2022 Document Type: Article