Biogenic nanosilver-fabricated endotracheal tube to prevent microbial colonization in a veterinary hospital.
Appl Microbiol Biotechnol
; 107(2-3): 623-638, 2023 Feb.
Article
in English
| MEDLINE | ID: covidwho-2268536
ABSTRACT
COVID-19 patients have often required prolonged endotracheal intubation, increasing the risk of developing ventilator-associated pneumonia (VAP). A preventive strategy is proposed based on an endotracheal tube (ETT) modified by the in situ deposition of eucalyptus-mediated synthesized silver nanoparticles (AgNPs). The surfaces of the modified ETT were embedded with AgNPs of approximately 28 nm and presented a nanoscale roughness. Energy dispersive X-ray spectroscopy confirmed the presence of silver on and inside the coated ETT, which exhibited excellent antimicrobial activity against Gram-positive and Gram-negative bacteria, and fungi, including multidrug-resistant clinical isolates. Inhibition of planktonic growth and microbial adhesion ranged from 99 to 99.999% without cytotoxic effects on mammalian cells. Kinetic studies showed that microbial adhesion to the coated surface was inhibited within 2 h. Cell viability in biofilms supplemented with human tracheal mucus was reduced by up to 95%. In a porcine VAP model, the AgNPs-coated ETT prevented adhesion of Pseudomonas aeruginosa and completely inhibited bacterial invasion of lung tissue. The potential antimicrobial efficacy and safety of the coated ETT were established in a randomized control trial involving 47 veterinary patients. The microbial burden was significantly lower on the surface of the AgNPs-coated ETT than on the uncoated ETT (p < 0.05). KEY POINTS ⢠Endotracheal tube surfaces were modified by coating with green-synthesized AgNPs ⢠P. aeruginosa burden of endotracheal tube and lung was reduced in a porcine model ⢠Effective antimicrobial activity and safety was demonstrated in a clinical trial.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Communicable Diseases
/
Pneumonia, Ventilator-Associated
/
Metal Nanoparticles
/
COVID-19
/
Anti-Infective Agents
Type of study:
Experimental Studies
/
Prognostic study
/
Randomized controlled trials
Limits:
Animals
/
Humans
Language:
English
Journal:
Appl Microbiol Biotechnol
Year:
2023
Document Type:
Article
Affiliation country:
S00253-022-12327-w
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