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Enhancing neutralizing antibodies against receptor binding domain of SARS-CoV-2 by a safe natural adjuvant system.
Darvish, Maliheh; Moosavi-Nejad, Zahra; Siadat, Seyed Omid Ranaei; Fatemi, Fataneh; Khatibi, Ali.
  • Darvish M; Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran. Electronic address: malihehdarvish67@gmail.com.
  • Moosavi-Nejad Z; Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran. Electronic address: z.moosavinejad@alzahra.ac.ir.
  • Siadat SOR; Protein Research Center, Shahid Beheshti University, Tehran, Iran. Electronic address: o_ranaei@sbu.ac.ir.
  • Fatemi F; Protein Research Center, Shahid Beheshti University, Tehran, Iran.
  • Khatibi A; Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Virus Res ; 326: 199047, 2023 03.
Article in English | MEDLINE | ID: covidwho-2184352
ABSTRACT
The receptor binding domain (RBD) plays a pivotal role in the viral entry as it enables the engagement of severe acute respiratory syndrome 2 (SARS-CoV-2) with the human angiotensin-converting enzyme 2 (ACE2) receptor for host cell entry. RBD is the major target for developing viral inhibitors and vaccines. Expression of recombinant RBD in E.coli is highly scalable with a low-cost procedure despite its high expression level compared to expression in mammalian and yeast cells. Using an alternative natural adjuvant system instead of alum adjuvant, increased immunogenicity of RBD antigen in serological assay including direct ELISA and surrogate Virus Neutralization Test (sVNT) was demonstrated with high levels of IgGs and neutralizing antibodies in mice sera immunized with RBDAlSa (Alum and Sodium alginate) formulation. The sVNT is a simple and fast test that can be used instead of the conventional virus neutralization test requiring live virus and BSL3 laboratory to detect total neutralizing antibodies against RBD. Additionally, results showed a safety profile for sodium alginate which supported using it as an alternative natural adjuvant.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Topics: Vaccines Limits: Animals / Humans Language: English Journal: Virus Res Journal subject: Virology Year: 2023 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Topics: Vaccines Limits: Animals / Humans Language: English Journal: Virus Res Journal subject: Virology Year: 2023 Document Type: Article