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Impedance-Based Neutralizing Antibody Detection Biosensor with Application in SARS-CoV-2 Infection.
Manshadi, Mohammad K D; Mansoorifar, Amin; Chiao, Jung-Chih; Beskok, Ali.
  • Manshadi MKD; Mechanical Engineering Department, Southern Methodist University, Dallas, Texas75275, United States.
  • Mansoorifar A; Mechanical Engineering Department, Southern Methodist University, Dallas, Texas75275, United States.
  • Chiao JC; Electrical and Computer Engineering Department, Southern Methodist University, Dallas, Texas75275, United States.
  • Beskok A; Mechanical Engineering Department, Southern Methodist University, Dallas, Texas75275, United States.
Anal Chem ; 95(2): 836-845, 2023 01 17.
Article in English | MEDLINE | ID: covidwho-2185423
ABSTRACT
Although safe and efficacious coronavirus disease-2019 (COVID-19) vaccines are available, real protective immunity is revealed by the serum COVID-19 neutralizing antibody (NAb) concentration. NAbs deactivate the virus by attaching to the viral receptor-binding domain (RBD), which interacts with angiotensin-converting enzyme 2 (ACE2) on the human cell. This paper introduces inexpensive, rapid, sensitive, and quantifiable impedance-based immunosensors to evaluate the NAb. The sensor limit of detection is experimentally determined in different buffer dilutions using bovine IgG-anti-bovine IgG interaction. The dominance of AC electrokinetic transport and molecular diffusion in the sensor is investigated using scaling analysis and numerical simulations. The results demonstrated that the sensor detection mechanism is mainly based on the diffusion of the biomolecules onto the electrode surface. After evaluating the sensor working principles, viral RBD buffers, including different NAb concentrations, are applied to the sensor, immobilized with the human ACE2 (hACE2). Results demonstrate that the sensor is capable of NAb detection in the analytical measuring interval between 45 ng/mL and 185 ng/mL. Since the present sensor provides fast test results with lower costs, it can be used to assess the NAb in people's blood serum before receiving further COVID vaccine doses.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Vaccines Limits: Humans Language: English Journal: Anal Chem Year: 2023 Document Type: Article Affiliation country: Acs.analchem.2c03193

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Vaccines Limits: Humans Language: English Journal: Anal Chem Year: 2023 Document Type: Article Affiliation country: Acs.analchem.2c03193