Rapid Detection of SARS-CoV-2 Spike RBD Protein in Body Fluid: Based on Special Calcium Ion-Mediated Gold Nanoparticles Modified by Bromide Ions.
J Phys Chem Lett
; 14(1): 88-94, 2023 Jan 12.
Article
in English
| MEDLINE | ID: covidwho-2185481
ABSTRACT
The receptor-binding domain of the SARS-CoV-2 spike mediates the key to binding the virus to the host receptor, but capturing the molecular signal of this spike RBD remains a formidable challenge. Here, we report a new surface-enhanced Raman spectroscopy (SERS) approach, which used gold nanoparticles prepared by low-speed constant-temperature centrifugation by bromine and calcium ions in two cleaning steps as the enhanced substrate to rapidly and accurately detect spike RBD large protein molecules in body fluids. The detection signal was extremely stable, and the orientation of the spike RBD on the enhanced substrate surface was also determined. This approach was specific in distinguishing different SARS-CoV-2 variants of spike RBD, including Delta, Beta, Gamma, and Omicron. Additionally, the enhanced substrate can identify biologically active or inactive spike RBD. This two-step cleaning enhanced substrate opens up opportunities not only for early diagnostics of SARS-CoV-2 virus but also for developing targeted drugs against viruses.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Body Fluids
/
Metal Nanoparticles
/
COVID-19
Type of study:
Diagnostic study
Topics:
Variants
Limits:
Humans
Language:
English
Journal:
J Phys Chem Lett
Year:
2023
Document Type:
Article
Affiliation country:
Acs.jpclett.2c03069
Similar
MEDLINE
...
LILACS
LIS