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Endotracheal tube biofilm in critically ill patients during the COVID-19 pandemic : description of an underestimated microbiological compartment.
Maldiney, Thomas; Pineau, Valentin; Neuwirth, Catherine; Ouzen, Linda; Eberl, Isabelle; Jeudy, Géraldine; Dalac, Sophie; Piroth, Lionel; Blot, Mathieu; Sautour, Marc; Dalle, Frédéric; Abdulmalak, Caroline; Ter Schiphorst, Romain; Pugliesi, Paul-Simon; Poussant, Thomas; Ogier-Desserrey, Agathe; Fournel, Isabelle; de Giraud d'Agay, Melchior; Jacquier, Marine; Labruyère, Marie; Aptel, François; Roudaut, Jean-Baptiste; Vieille, Thibault; Andreu, Pascal; Prin, Sébastien; Charles, Pierre-Emmanuel; Hamet, Maël; Quenot, Jean-Pierre.
  • Maldiney T; Department of Intensive Care Medicine, William Morey General Hospital, Chalon-Sur-Saône, France. thomas.maldiney@ch-chalon71.fr.
  • Pineau V; Lipness Team, INSERM Research Centre LNC-UMR1231 and LabEx LipSTIC, University Bourgogne-Franche-Comté, Dijon, France. thomas.maldiney@ch-chalon71.fr.
  • Neuwirth C; Infectious Diseases Department, University Hospital of Dijon, Dijon, France.
  • Ouzen L; Department of Bacteriology, University Hospital of Dijon, Dijon, France.
  • Eberl I; UMR/CNRS 6249 Chrono-Environnement, University Bourgogne Franche-Comté, Besançon, France.
  • Jeudy G; Department of Bacteriology, University Hospital of Dijon, Dijon, France.
  • Dalac S; Infectious Diseases Department, University Hospital of Dijon, Dijon, France.
  • Piroth L; Dermatology Department, University Hospital of Dijon, Dijon, France.
  • Blot M; Dermatology Department, University Hospital of Dijon, Dijon, France.
  • Sautour M; Infectious Diseases Department, University Hospital of Dijon, Dijon, France.
  • Dalle F; Centre d'Investigation Clinique, CHU Dijon; INSERM, CIC 1432, Module Epidémiologie Clinique, CHU Dijon, Dijon, France.
  • Abdulmalak C; Lipness Team, INSERM Research Centre LNC-UMR1231 and LabEx LipSTIC, University Bourgogne-Franche-Comté, Dijon, France.
  • Ter Schiphorst R; Infectious Diseases Department, University Hospital of Dijon, Dijon, France.
  • Pugliesi PS; Department of Parasitology/Mycology, University Hospital of Dijon, Dijon, France.
  • Poussant T; Department of Parasitology/Mycology, University Hospital of Dijon, Dijon, France.
  • Ogier-Desserrey A; UMR PAM A 02.102 Procédés Alimentaires et Microbiologiques, University Bourgogne Franche-Comté, Agrosup Dijon, Dijon, France.
  • Fournel I; Department of Intensive Care Medicine, William Morey General Hospital, Chalon-Sur-Saône, France.
  • de Giraud d'Agay M; Department of Intensive Care Medicine, William Morey General Hospital, Chalon-Sur-Saône, France.
  • Jacquier M; Department of Intensive Care Medicine, William Morey General Hospital, Chalon-Sur-Saône, France.
  • Labruyère M; Department of Intensive Care Medicine, William Morey General Hospital, Chalon-Sur-Saône, France.
  • Aptel F; Laboratoire de Biologie Médicale, William Morey General Hospital, Chalon-Sur-Saône, France.
  • Roudaut JB; Centre d'Investigation Clinique, CHU Dijon; INSERM, CIC 1432, Module Epidémiologie Clinique, CHU Dijon, Dijon, France.
  • Vieille T; Centre d'Investigation Clinique, CHU Dijon; INSERM, CIC 1432, Module Epidémiologie Clinique, CHU Dijon, Dijon, France.
  • Andreu P; Department of Intensive Care Medicine, University Hospital of Dijon, Dijon, France.
  • Prin S; Department of Intensive Care Medicine, University Hospital of Dijon, Dijon, France.
  • Charles PE; Department of Intensive Care Medicine, University Hospital of Dijon, Dijon, France.
  • Hamet M; Department of Intensive Care Medicine, University Hospital of Dijon, Dijon, France.
  • Quenot JP; Department of Intensive Care Medicine, University Hospital of Dijon, Dijon, France.
Sci Rep ; 12(1): 22389, 2022 12 27.
Article in English | MEDLINE | ID: covidwho-2186041
ABSTRACT
Biofilm (BF) growth is believed to play a major role in the development of ventilator-associated pneumonia (VAP) in the intensive care unit. Despite concerted efforts to understand the potential implication of endotracheal tube (ETT)-BF dispersal, clinically relevant data are lacking to better characterize the impact of its mesostructure and microbiological singularity on the occurrence of VAP. We conducted a multicenter, retrospective observational study during the third wave of the COVID-19 pandemic, between March and May 2021. In total, 64 ETTs collected from 61 patients were included in the present BIOPAVIR study. Confocal microscopy acquisitions revealed two main morphological aspects of ETT-deposited BF (1) a thin, continuous ribbon-shaped aspect, less likely monobacterial and predominantly associated with Enterobacter spp., Streptococcus pneumoniae or Viridans streptococci, and (2) a thicker, discontinuous, mushroom-shaped appearance, more likely characterized by the association of bacterial and fungal species in respiratory samples. The microbiological characterization of ETT-deposited BF found higher acquired resistance in more than 80% of analyzed BF phenotypes, compared to other colonization sites from the patient's environment. These findings reveal BF as a singular microbiological compartment, and are of added clinical value, with a view to future ETT-deposited BF-based antimicrobial stewardship in critically ill patients. Trial registration NCT04926493. Retrospectively registered 15 June 2021.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Ventilator-Associated / COVID-19 Type of study: Observational study / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Sci Rep Year: 2022 Document Type: Article Affiliation country: S41598-022-26560-w

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Ventilator-Associated / COVID-19 Type of study: Observational study / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Sci Rep Year: 2022 Document Type: Article Affiliation country: S41598-022-26560-w