A Monoclonal Antibody Targeting C-Terminal Domain of Transmissible Gastroenteritis Virus Spike Protein.
Monoclon Antib Immunodiagn Immunother
; 41(6): 328-332, 2022 Dec.
Article
in English
| MEDLINE | ID: covidwho-2188107
ABSTRACT
The structure and function of the C-terminus domain (CTD) of porcine transmissible gastroenteritis virus (TGEV) spike protein remain largely unknown, thereby a specific monoclonal antibody (MAb) allows us to fully understand this domain. In this study, we developed a murine MAb against CTD of TGEV spike protein, as evidenced by the results of indirect fluorescent assay, Western blotting, and fluorescence-activated cell sorter. Further study showed that the MAb is able to exclusively recognize a 12-residue peptide (FKNVSDGVIYSV) derived from CTD of TGEV spike protein. This MAb can be used to elucidate the potential function of CTD of TGEV spike in virus attachment and entry, and warrants further intensive investigation.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Transmissible gastroenteritis virus
/
Gastroenteritis, Transmissible, of Swine
Limits:
Animals
Language:
English
Journal:
Monoclon Antib Immunodiagn Immunother
Year:
2022
Document Type:
Article
Affiliation country:
Mab.2022.0030
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