Lab-on-PCB-Based Electrical Immunosensing Platform for Point-of-Care Detection of SARS-CoV-2
Ieee Sensors Letters
; 7(1), 2023.
Article
in English
| Web of Science | ID: covidwho-2192007
ABSTRACT
The present work shows a multiplexed lab-on-printed circuit board (PCB) platform for label-free immunosensing of SARS-CoV-2 nucleocapsid and spike antigens based on electrical impedance measurements. The sensor consists of an interdigitated electrode of soft gold integrated on a PCB with microwells for sample loading. A mercaptoundecanoic acid-protein-G-based site-specific biofunctionalization strategy is employed to efficiently immobilize dual antibodies on the device surface toward the sensitive and rapid antigen test of SARS-CoV-2. Electrical impedance measurements carried out in a point-of-care setting using the PalmSens Sensit Smart system that could detect nucleocapsid and spike proteins with a detection limit of 40 and 20 pg each. Experiments with nasopharyngeal swab samples from N = 5 healthy and N = 14 SARS-CoV-2 positive subjects showed significantly different electrical responses for subjects with high viral load (Ct < 25) compared with healthy subjects and control.
Full text:
Available
Collection:
Databases of international organizations
Database:
Web of Science
Language:
English
Journal:
Ieee Sensors Letters
Year:
2023
Document Type:
Article
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