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Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors.
Ning, Kang; Zou, Wei; Xu, Peng; Cheng, Fang; Zhang, Elizabeth Yan; Zhang-Chen, Aaron; Kleiboeker, Steve; Qiu, Jianming.
  • Ning K; Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
  • Zou W; Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
  • Xu P; Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
  • Cheng F; Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
  • Zhang EY; GeneGoCell Inc., San Diego, CA 92127, USA.
  • Zhang-Chen A; GeneGoCell Inc., San Diego, CA 92127, USA.
  • Kleiboeker S; Department of Research and Development, ViraCor Eurofins Laboratories, Lenexa, KS 66219, USA.
  • Qiu J; Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
Sci Adv ; 9(2): eade0869, 2023 01 13.
Article in English | MEDLINE | ID: covidwho-2193381
ABSTRACT
Parvovirus B19 (B19V) infects human erythroid progenitor cells (EPCs) and causes several hematological disorders and fetal hydrops. Amino acid (aa) 5-68 of minor capsid protein VP1 (VP1u5-68aa) is the minimal receptor binding domain for B19V to enter EPCs. Here, we carried out a genome-wide CRISPR-Cas9 guide RNA screen and identified tyrosine protein kinase receptor UFO (AXL) as a proteinaceous receptor for B19V infection of EPCs. AXL gene silencing in ex vivo expanded EPCs remarkably decreased B19V internalization and replication. Additions of the recombinant AXL extracellular domain or a polyclonal antibody against it upon infection efficiently inhibited B19V infection of ex vivo expanded EPCs. Moreover, B19V VP1u interacted with the recombinant AXL extracellular domain in vitro at a relatively high affinity (KD = 103 nM). Collectively, we provide evidence that AXL is a co-receptor for B19V infection of EPCs.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Parvovirus B19, Human / Erythema Infectiosum / Axl Receptor Tyrosine Kinase Type of study: Diagnostic study Limits: Humans Language: English Journal: Sci Adv Year: 2023 Document Type: Article Affiliation country: Sciadv.ade0869

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Parvovirus B19, Human / Erythema Infectiosum / Axl Receptor Tyrosine Kinase Type of study: Diagnostic study Limits: Humans Language: English Journal: Sci Adv Year: 2023 Document Type: Article Affiliation country: Sciadv.ade0869