Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors.
Sci Adv
; 9(2): eade0869, 2023 01 13.
Article
in English
| MEDLINE | ID: covidwho-2193381
ABSTRACT
Parvovirus B19 (B19V) infects human erythroid progenitor cells (EPCs) and causes several hematological disorders and fetal hydrops. Amino acid (aa) 5-68 of minor capsid protein VP1 (VP1u5-68aa) is the minimal receptor binding domain for B19V to enter EPCs. Here, we carried out a genome-wide CRISPR-Cas9 guide RNA screen and identified tyrosine protein kinase receptor UFO (AXL) as a proteinaceous receptor for B19V infection of EPCs. AXL gene silencing in ex vivo expanded EPCs remarkably decreased B19V internalization and replication. Additions of the recombinant AXL extracellular domain or a polyclonal antibody against it upon infection efficiently inhibited B19V infection of ex vivo expanded EPCs. Moreover, B19V VP1u interacted with the recombinant AXL extracellular domain in vitro at a relatively high affinity (KD = 103 nM). Collectively, we provide evidence that AXL is a co-receptor for B19V infection of EPCs.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Parvovirus B19, Human
/
Erythema Infectiosum
/
Axl Receptor Tyrosine Kinase
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Sci Adv
Year:
2023
Document Type:
Article
Affiliation country:
Sciadv.ade0869
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