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Impact of Temporary Storage Conditions on the Viability of Streptococcus pneumoniae in Saliva.
Allicock, Orchid M; York, Anna; Waghela, Pari; Yolda-Carr, Devyn; Weinberger, Daniel M; Wyllie, Anne L.
  • Allicock OM; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, USA.
  • York A; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, USA.
  • Waghela P; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, USA.
  • Yolda-Carr D; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, USA.
  • Weinberger DM; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, USA.
  • Wyllie AL; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, USA.
mSphere ; : e0033122, 2022 Nov 21.
Article in English | MEDLINE | ID: covidwho-2193480
ABSTRACT
Nasopharyngeal swabs are considered the gold-standard sample type for the detection of Streptococcus pneumoniae carriage, but recent studies have demonstrated the utility of saliva in improving the detection of carriage in adults. Saliva is generally collected in its raw, unsupplemented state, unlike nasopharyngeal swabs, which are collected into stabilizing transport media. Few data exist regarding the stability of pneumococci in unsupplemented saliva during transport and laboratory storage. We therefore evaluated the effect of storage conditions on the detection of pneumococci in saliva samples using strains representing eight pneumococcal serotypes. The bacteria were spiked into raw saliva from asymptomatic individuals, and we assessed sample viability after storage at 4°C, room temperature, and 30°C for up to 72 h; at 40°C for 24 h; and following three freeze-thaw cycles. We observed little decrease in pneumococcal detection following culture enrichment and quantitative PCR (qPCR) detection of the piaB and lytA genes compared to testing fresh samples, indicating the prolonged viability of pneumococci in neat saliva samples. This sample stability makes saliva a viable sample type for pneumococcal carriage studies conducted in remote or low-resource settings and provides insight into the effect of the storage of saliva samples in the laboratory. IMPORTANCE For pneumococcal carriage studies, saliva is a sample type that can overcome some of the issues typically seen with nasopharyngeal and oropharyngeal swabs. Understanding the limitations of saliva as a sample type is important for maximizing its use. This study sought to better understand how different storage conditions and freeze-thaw cycles affect pneumococcal survival over time. These findings support the use of saliva as an alternative sample type for pneumococcal carriage studies, particularly in remote or low-resource settings with reduced access to health care facilities.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study Language: English Journal: MSphere Year: 2022 Document Type: Article Affiliation country: Msphere.00331-22

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study Language: English Journal: MSphere Year: 2022 Document Type: Article Affiliation country: Msphere.00331-22