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Assessment of Immunogenic and Antigenic Properties of Recombinant Nucleocapsid Proteins of Five SARS-CoV-2 Variants in a Mouse Model.
Rak, Alexandra; Gorbunov, Nikolay; Kostevich, Valeria; Sokolov, Alexey; Prokopenko, Polina; Rudenko, Larisa; Isakova-Sivak, Irina.
  • Rak A; Department of Virology, Institute of Experimental Medicine, Saint Petersburg 197022, Russia.
  • Gorbunov N; Department of Molecular Genetics, Institute of Experimental Medicine, Saint Petersburg 197022, Russia.
  • Kostevich V; Department of Molecular Genetics, Institute of Experimental Medicine, Saint Petersburg 197022, Russia.
  • Sokolov A; Department of Molecular Genetics, Institute of Experimental Medicine, Saint Petersburg 197022, Russia.
  • Prokopenko P; Department of Virology, Institute of Experimental Medicine, Saint Petersburg 197022, Russia.
  • Rudenko L; Department of Virology, Institute of Experimental Medicine, Saint Petersburg 197022, Russia.
  • Isakova-Sivak I; Department of Virology, Institute of Experimental Medicine, Saint Petersburg 197022, Russia.
Viruses ; 15(1)2023 Jan 13.
Article in English | MEDLINE | ID: covidwho-2200881
ABSTRACT
COVID-19 cases caused by new variants of highly mutable SARS-CoV-2 continue to be identified worldwide. Effective control of the spread of new variants can be achieved through targeting of conserved viral epitopes. In this regard, the SARS-CoV-2 nucleocapsid (N) protein, which is much more conserved than the evolutionarily influenced spike protein (S), is a suitable antigen. The recombinant N protein can be considered not only as a screening antigen but also as a basis for the development of next-generation COVID-19 vaccines, but little is known about induction of antibodies against the N protein via different SARS-CoV-2 variants. In addition, it is important to understand how antibodies produced against the antigen of one variant can react with the N proteins of other variants. Here, we used recombinant N proteins from five SARS-CoV-2 strains to investigate their immunogenicity and antigenicity in a mouse model and to obtain and characterize a panel of hybridoma-derived monoclonal anti-N antibodies. We also analyzed the variable epitopes of the N protein that are potentially involved in differential recognition of antiviral antibodies. These results will further deepen our knowledge of the cross-reactivity of the humoral immune response in COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Observational study / Randomized controlled trials Topics: Vaccines / Variants Limits: Animals / Humans Language: English Year: 2023 Document Type: Article Affiliation country: V15010230

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Observational study / Randomized controlled trials Topics: Vaccines / Variants Limits: Animals / Humans Language: English Year: 2023 Document Type: Article Affiliation country: V15010230