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Expanding the Pathogen Panel in Wastewater Epidemiology to Influenza and Norovirus.
Markt, Rudolf; Stillebacher, Fabian; Nägele, Fabiana; Kammerer, Anna; Peer, Nico; Payr, Maria; Scheffknecht, Christoph; Dria, Silvina; Draxl-Weiskopf, Simon; Mayr, Markus; Rauch, Wolfgang; Kreuzinger, Norbert; Rainer, Lukas; Bachner, Florian; Zuba, Martin; Ostermann, Herwig; Lackner, Nina; Insam, Heribert; Wagner, Andreas Otto.
  • Markt R; Department of Microbiology, Universität Innsbruck, 6020 Innsbruck, Austria.
  • Stillebacher F; Department of Health Sciences and Social Work, Carinthia University of Applied Sciences, 9020 Klagenfurt, Austria.
  • Nägele F; Department of Microbiology, Universität Innsbruck, 6020 Innsbruck, Austria.
  • Kammerer A; Department of Microbiology, Universität Innsbruck, 6020 Innsbruck, Austria.
  • Peer N; Department of Microbiology, Universität Innsbruck, 6020 Innsbruck, Austria.
  • Payr M; Department of Microbiology, Universität Innsbruck, 6020 Innsbruck, Austria.
  • Scheffknecht C; Department of Microbiology, Universität Innsbruck, 6020 Innsbruck, Austria.
  • Dria S; Institut für Umwelt und Lebensmittelsicherheit des Landes Vorarlberg, 6900 Bregenz, Austria.
  • Draxl-Weiskopf S; Institut für Umwelt und Lebensmittelsicherheit des Landes Vorarlberg, 6900 Bregenz, Austria.
  • Mayr M; Department of Microbiology, Universität Innsbruck, 6020 Innsbruck, Austria.
  • Rauch W; Department of Microbiology, Universität Innsbruck, 6020 Innsbruck, Austria.
  • Kreuzinger N; Department of Infrastructure, Universität Innsbruck, 6020 Innsbruck, Austria.
  • Rainer L; Institute for Water Quality and Resource Management, Technische Universität Wien, 1040 Vienna, Austria.
  • Bachner F; Austrian National Public Health Institute, 1010 Vienna, Austria.
  • Zuba M; Austrian National Public Health Institute, 1010 Vienna, Austria.
  • Ostermann H; Austrian National Public Health Institute, 1010 Vienna, Austria.
  • Lackner N; Austrian National Public Health Institute, 1010 Vienna, Austria.
  • Insam H; Department of Health Sciences and Social Work, Carinthia University of Applied Sciences, 9020 Klagenfurt, Austria.
  • Wagner AO; Department of Microbiology, Universität Innsbruck, 6020 Innsbruck, Austria.
Viruses ; 15(2)2023 01 17.
Article in English | MEDLINE | ID: covidwho-2270934
ABSTRACT
Since the start of the 2019 pandemic, wastewater-based epidemiology (WBE) has proven to be a valuable tool for monitoring the prevalence of SARS-CoV-2. With methods and infrastructure being settled, it is time to expand the potential of this tool to a wider range of pathogens. We used over 500 archived RNA extracts from a WBE program for SARS-CoV-2 surveillance to monitor wastewater from 11 treatment plants for the presence of influenza and norovirus twice a week during the winter season of 2021/2022. Extracts were analyzed via digital PCR for influenza A, influenza B, norovirus GI, and norovirus GII. Resulting viral loads were normalized on the basis of NH4-N. Our results show a good applicability of ammonia-normalization to compare different wastewater treatment plants. Extracts originally prepared for SARS-CoV-2 surveillance contained sufficient genomic material to monitor influenza A, norovirus GI, and GII. Viral loads of influenza A and norovirus GII in wastewater correlated with numbers from infected inpatients. Further, SARS-CoV-2 related non-pharmaceutical interventions affected subsequent changes in viral loads of both pathogens. In conclusion, the expansion of existing WBE surveillance programs to include additional pathogens besides SARS-CoV-2 offers a valuable and cost-efficient possibility to gain public health information.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Norovirus / Influenza, Human / COVID-19 Type of study: Observational study Limits: Humans Language: English Year: 2023 Document Type: Article Affiliation country: V15020263

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Norovirus / Influenza, Human / COVID-19 Type of study: Observational study Limits: Humans Language: English Year: 2023 Document Type: Article Affiliation country: V15020263