Establishment and evaluation of fluorescent RT-RAA assay for SARS-CoV-2 sgRNAs detection
Zhongguo Bingdubing Zazhi = Chinese Journal of Viral Diseases
; 12(6):444, 2022.
Article
in Chinese
| ProQuest Central | ID: covidwho-2204245
ABSTRACT
Objective To establish a reverse-transcription recombinase-aided amplification assay(RT-RAA) to rapidly detect SARS-CoV-2 sub-genomic RNAs(sgRNAs). Methods The primers and probe for isothermal nucleic acid amplification were designed based on the 5′-leader and 7a and N gene sequence of SARS-CoV-2, and the sgRNAs of SARS-COV-2 were rapidly detected within 30 min at 39 ℃.The sensitivity, specificity and consistency of the assay were evaluated. Results The detection limit of the method was 20 copies/μl and there were no cross-reactions with other respiratory pathogens, showing decent sensitivity and specificity.The results of the assay were concordant with that of real-time PCR, indicating a better consistency of two methods(κ=0.762,P<0.001). Conclusions The fluorescence RT-RAA assay established in the study can be used for the rapid detection of SARS-CoV-2 sgRNAs, which is of great significance for the rapid diagnosis of COVID 19.
Full text:
Available
Collection:
Databases of international organizations
Database:
ProQuest Central
Type of study:
Experimental Studies
Language:
Chinese
Journal:
Zhongguo Bingdubing Zazhi = Chinese Journal of Viral Diseases
Year:
2022
Document Type:
Article
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