Simultaneous Detection of Eight Antiviral Drugs for Treatment of COVID-19 in Source Water

ABSTRACT

The continuous outbreak of COVID-19 results in a large number of therapeutic drugs inevitably entering water bodies through different pathways, and there are problems of accurate and rapid detection in the treatment and ecological evaluation of these drugs. Therefore, it is necessary to establish a method for simultaneous detection of multiple antiviral drugs for treatment of COVID-19 in water. A method for the simultaneous detection of 8 antiviral drugs（ribavirin, oseltamivir, nevirapine, lamivudine,abacavir, stavudine, acyclovir and penciclovir） in source water was developed by optimizing the determining parameters of liquid chromatography-tandem mass spectrometry（LC-MS/MS）. The daughter ion response values of 8 antiviral drugs in this method were relatively high, which was beneficial to the detection of target compounds. The correlation coefficient（r） of the standard curve of 8 antiviral drugs was not less than 0.995 0, the method detection limit（MDL） and the lower limit of determination were in the range of 0.004-0.081 μg/L and 0.013-0.267 μg/L, respectively, and the spike recoveries and RSDs of actual water samples were 81.61%-113.03% and 0.84%-8.12%, respectively.