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Molecular Medical Diagnosis of COVID-19 and Omicron Variant
Journal of Pharmaceutical Negative Results ; 13:6332-6347, 2022.
Article in English | EMBASE | ID: covidwho-2206806
ABSTRACT
Genetic lineages of severe acute respiratory syndrome corona virus-2 (SARS-CoV-2) have continued to emerge and circulate around the world since the onset of the COVID-19 pandemic. There are numerous variants of SARS-CoV-2, the virus that causes corona virus disease 2019 (COVID-19). Like other viruses, SARS-CoV-2 evolves over time. Most mutations in the SARS-CoV-2 genome have no impact on viral function, but certain variants have gained worldwide attention because of their rapid emergence within populations, evidence of transmission, and clinical implications. During the pandemic, most parts of India were affected, including Odisha, leading to high rates of morbidity and mortality. For the present study, 368,303 samples were received by the COVID-19 lab i.e., medical (Virus Research Diagnostic Laboratory) VRDL from six districts of western Odisha, including approximately 25,000 COVID-19-positive samples. The diagnostic method of the quantitative RT-PCR cannot be used to distinguish among the variants created by mutation of the genes initially. Therefore, selected positive clinical samples were sent in cold chain for whole genome sequencing (WGS), and disease severity was sequenced using the Illumina Seq at ILS, BBSR for variant detection. The reported observation from the next generation sequencing (NGS) based sequenced samples of western Odisha updated in the INSACOG-WGS portal confirms the presence of Delta (B.1.617.2) and Delta sub lineages, Omicron (BA.2), and Omicron (B.1.1.529). Maximum infection was caused by Delta sub lineages 83.5%) irrespective of age, sex, and geographic area followed by Delta and Omicron. Molecular diagnosis and WGS based study reveal the widespread transmission of the fatal virus, significantly affecting every corner of the globe. Copyright © 2022 Wolters Kluwer Medknow Publications. All rights reserved.
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Full text: Available Collection: Databases of international organizations Database: EMBASE Topics: Variants Language: English Journal: Journal of Pharmaceutical Negative Results Year: 2022 Document Type: Article

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Full text: Available Collection: Databases of international organizations Database: EMBASE Topics: Variants Language: English Journal: Journal of Pharmaceutical Negative Results Year: 2022 Document Type: Article