Assessment of Cinamic acid and Costus roots extract against MDR-K. pneumoniae isolated from patients of COVID-19

ABSTRACT

The emergence of resistant Klebsiella pneumoniae associated with COVID-19 demonstrate a primary challenge for the antimicrobial therapy of infectious diseases and increases the incidence of mortality and morbidity." K. pneumoniae isolated from COVID-19 patient's sputum with ratio (100%). All K. pneumoniae clinical isolates had 100% resistance to ceftriaxone, piperacillin (80%), cefepime (60%), amikacin (40%), and meropenem =levofloxccin (20%). Bacterial isolates gave positive result for mCIM with ratio was 100%, also all isolates produced diversity of beta-lactamases at a rate of 100% by using spectrometry beta-lactamase assay. Costunolide (38.3 %), Rutin (15.33%), Pentadecanoic acid (6.54%), Oliec acid (4.77%), and Caproic acid (3.22%) considered as major compounds in Saussurea costus were identified by GC-Mass spectrometry. The beta-lactamase produced by K. pneumoniae were inhibited by Saussurea costus with a strong statistical significance at P-value <0.01, while cinamic acid donot effected on beta-lactamase activity. Copyright © RJPT All right reserved.