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PLANT HSP90S AS NOVEL ADJUVANTS in the SARS-COV2 MODEL: HUMORAL RESPONSE ANALYSIS and ANTIBODY NEUTRALIZATION ASSAY in MICE
Biocell ; 47(Supplement 1):200, 2023.
Article in English | EMBASE | ID: covidwho-2207924
ABSTRACT
Heat shock proteins of 90 kDa (HSP90) have been proposed as adjuvants in the design of new vaccine formulations. In our laboratory, two cytosolic isoforms of plant HSP90 (AtHsp81.2 from Arabidopsis thaliana and NbHsp90.3 from Nicotiana benthamiana) demonstrate to have adjuvant properties against parasitic infections such as Toxoplasmosis and Neosporosis. We showed that plant HSP90 fused or mixed with the antigen of interest can enhance and modulate the immune response. To evaluate the adjuvant properties of plant HSP90 in other infectious diseases poorly characterized, we proposed to use the receptor binding domain (RBD) of the Spike protein of SARS-CoV2, and the main candidate in the design of subunit vaccines. Initially, we evaluated the humoral response against RBD using the strategy protein mixture (Adjuvant + Antigen). Thirty C57 mice (male and female) were randomly separated into 7 groups and intramuscularly immunized with AtHsp81.2, NbHsp90.3 or RBD as control groups, rRBD + rAtHsp81.2, rRBD + rNbHsp90.3 or rRBD + Alum as vaccinated groups. A PBS group immunized with PBS 1x buffer was also included. Mice received a two-dose schedule delivered in 21-day intervals, and the sera were obtained at 0, 21-, 42-, 63-and 84-days post-immunization (dpi). The analysis of the humoral response showed a significant increase in anti-RBD IgGt, which persisted between 21 and 42 dpi. In addition, the RBD + AtHsp81.2 group showed a Th2 profile with a significant increase of anti-RBD IgG1 like the control RBD + Alum group. By contrast, the RBD + NbHsp90.3 group showed a significant increase in anti-RBD IgG2b towards a Th1 profile. Finally, we evaluated the capacity of the sera to neutralize lentiviral vectors pseudotyped with Spike glycoprotein. Serum-neutralizing antibody titers were determined by the 50% inhibitory dilution. The sera obtained at 42 dpi from mice immunized with rRBD + rAtHsp81.2 and rRBD + rNbHsp90.3 showed the potential to neutralize viral infection. In addition, the differential profile of both isoforms in the triggered humoral response offers an advantage of rapid and safe response in pandemic situations such as those experienced by SARS-CoV2. Neurosciences.
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Collection: Databases of international organizations Database: EMBASE Language: English Journal: Biocell Year: 2023 Document Type: Article

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Collection: Databases of international organizations Database: EMBASE Language: English Journal: Biocell Year: 2023 Document Type: Article