Unable to write in log file ../../bases/logs/gimorg/logerror.txt Flexible Label-Free Platinum and Bio-PET-Based Immunosensor for the Detection of SARS-CoV-2. | Biosensors (Basel);13(2)2023 Jan 26. | MEDLINE
Flexible Label-Free Platinum and Bio-PET-Based Immunosensor for the Detection of SARS-CoV-2.
Blasques, Rodrigo Vieira; de Oliveira, Paulo Roberto; Kalinke, Cristiane; Brazaca, Laís Canniatti; Crapnell, Robert D; Bonacin, Juliano Alves; Banks, Craig E; Janegitz, Bruno Campos.
  • Blasques RV; Laboratory of Sensors, Nanomedicine and Nanostructured Materials, Federal University of São Carlos, Araras 13600-970, Brazil.
  • de Oliveira PR; Department of Physics, Chemistry, and Mathematics, Federal University of São Carlos, Sorocaba 18052-780, Brazil.
  • Kalinke C; Laboratory of Sensors, Nanomedicine and Nanostructured Materials, Federal University of São Carlos, Araras 13600-970, Brazil.
  • Brazaca LC; Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK.
  • Crapnell RD; Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK.
  • Bonacin JA; Institute of Chemistry, University of Campinas, Campinas 13083-970, Brazil.
  • Banks CE; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
  • Janegitz BC; Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK.
Biosensors (Basel) ; 13(2)2023 Jan 26.
Article in English | MEDLINE | ID: covidwho-2215583
ABSTRACT
The demand for new devices that enable the detection of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) at a relatively low cost and that are fast and feasible to be used as point-of-care is required overtime on a large scale. In this sense, the use of sustainable materials, for example, the bio-based poly (ethylene terephthalate) (Bio-PET) can be an alternative to current standard diagnostics. In this work, we present a flexible disposable printed electrode based on a platinum thin film on Bio-PET as a substrate for the development of a sensor and immunosensor for the monitoring of COVID-19 biomarkers, by the detection of L-cysteine and the SARS-CoV-2 spike protein, respectively. The electrode was applied in conjunction with 3D printing technology to generate a portable and easy-to-analyze device with a low sample volume. For the L-cysteine determination, chronoamperometry was used, which achieved two linear dynamic ranges (LDR) of 3.98-39.0 µmol L-1 and 39.0-145 µmol L-1, and a limit of detection (LOD) of 0.70 µmol L-1. The detection of the SARS-CoV-2 spike protein was achieved by both square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS) by a label-free immunosensor, using potassium ferro-ferricyanide solution as the electrochemical probe. An LDR of 0.70-7.0 and 1.0-30 pmol L-1, with an LOD of 0.70 and 1.0 pmol L-1 were obtained by SWV and EIS, respectively. As a proof of concept, the immunosensor was successfully applied for the detection of the SARS-CoV-2 spike protein in enriched synthetic saliva samples, which demonstrates the potential of using the proposed sensor as an alternative platform for the diagnosis of COVID-19 in the future.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Year: 2023 Document Type: Article Affiliation country: Bios13020190

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Year: 2023 Document Type: Article Affiliation country: Bios13020190