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The BNT162b2 mRNA COVID-19 Vaccine Increases the Contractile Sensitivity to Histamine and Parasympathetic Activation in a Human Ex Vivo Model of Severe Eosinophilic Asthma.
Calzetta, Luigino; Chetta, Alfredo; Aiello, Marina; Frizzelli, Annalisa; Ora, Josuel; Melis, Enrico; Facciolo, Francesco; Ippoliti, Lorenzo; Magrini, Andrea; Rogliani, Paola.
  • Calzetta L; Department of Medicine and Surgery, University of Parma, 43121 Parma, Italy.
  • Chetta A; Department of Medicine and Surgery, University of Parma, 43121 Parma, Italy.
  • Aiello M; Department of Medicine and Surgery, University of Parma, 43121 Parma, Italy.
  • Frizzelli A; Department of Medicine and Surgery, University of Parma, 43121 Parma, Italy.
  • Ora J; Department of Experimental Medicine, University of Rome "Tor Vergata", 00133 Rome, Italy.
  • Melis E; Thoracic Surgery Unit, Regina Elena National Cancer Institute, 00144 Rome, Italy.
  • Facciolo F; Thoracic Surgery Unit, Regina Elena National Cancer Institute, 00144 Rome, Italy.
  • Ippoliti L; Department of Biomedicine and Prevention, University of Rome "Tor Vergata", 00133 Rome, Italy.
  • Magrini A; Department of Biomedicine and Prevention, University of Rome "Tor Vergata", 00133 Rome, Italy.
  • Rogliani P; Department of Experimental Medicine, University of Rome "Tor Vergata", 00133 Rome, Italy.
Vaccines (Basel) ; 11(2)2023 Jan 28.
Article in English | MEDLINE | ID: covidwho-2217110
ABSTRACT
The BNT162b2 COVID-19 vaccine is composed of lipid-nanoparticles (LNP) containing the mRNA that encodes for SARS-CoV-2 spike glycoprotein. Bronchospasm has been reported as an early reaction after COVID-19 mRNA vaccines in asthmatic patients. The aim of this study was to investigate the acute impact of BNT162b2 in a human ex vivo model of severe eosinophilic asthma. Passively sensitized human isolated bronchi were challenged with the platelet-activating factor to reproduce ex vivo the hyperresponsiveness of airways of patients suffering from severe eosinophilic asthma. BNT162b2 was tested on the contractile sensitivity to histamine and parasympathetic activation via electrical field stimulation (EFS); some experiments were performed after mRNA denaturation. BNT162b2 increased the resting tone (+11.82 ± 2.27%) and response to histamine in partially contracted tissue (+42.97 ± 9.64%) vs. the control (p < 0.001); it also shifted the concentration-response curve to histamine leftward (0.76 ± 0.09 logarithm) and enhanced the response to EFS (+28.46 ± 4.40%) vs. the control. Denaturation did not significantly modify (p > 0.05) the effect of BNT162b2. BNT162b2 increases the contractile sensitivity to histamine and parasympathetic activation in hyperresponsive airways, a detrimental effect not related to the active component but to some excipient. A possible candidate for the bronchospasm elicited by BNT162b2 could be the polyethylene glycol/macrogol used to produce LNP.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Prognostic study Topics: Vaccines Language: English Year: 2023 Document Type: Article Affiliation country: Vaccines11020282

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Prognostic study Topics: Vaccines Language: English Year: 2023 Document Type: Article Affiliation country: Vaccines11020282