Your browser doesn't support javascript.
Nanosized graphite encapsulating technetium-99m metal complexes for lung ventilation studies in rats
European Journal of Nuclear Medicine and Molecular Imaging ; 49(Supplement 1):S449, 2022.
Article in English | EMBASE | ID: covidwho-2219997
ABSTRACT
Aim/

Introduction:

Many diseases with high prevalence and socioeconomic impact are caused by, or associated with, altered lung ventilation patterns. These include chronic obstructive pulmonary disease, COVID-19 infection, asthma, or lung cancer. SPECT/ CT images using a dispersion of nanosized (30-60nm) graphite encapsulating technetium-99m metal NG-eTcM (such as Technegas) are widely used to assess regional lung ventilation in the clinical setting. However, evaluation of lung ventilation in experimental animals has been barely explored. The main objective of this study was to develop and construct a system to deliver NG-eTcM to rats and obtain high resolution microSPECT/CT images of the lungs after administration of the radiotracer. Material(s) and Method(s) To adapt the human administration system for rats, a special chamber was designed, in which NG-eTcM was delivered from the NG-eTcM generator. The chamber was designed with pressure gauges, inlet and outlet checkvalves, a vacuum system to simulate deep inhalation and 3D-printend nozzles to place up to two anesthetised rats with their snouts into the chamber to inhale the technetiumgraphite suspension. NG-eTcM was prepared according to the manufacturer instructions (370 MBq in 0,1 mL) from a fresh eluate of a 99Mo/99mTc generator. Anesthetized Wistar rats were placed with the snout into the inhalation chamber for 10 minutes and images were obtained 12 and 50min post-administration in a U-SPECT6/Eclass microSPECT/CT scanner. SPECT images were reconstructed and attenuation correction applied using the CT attenuation map. SPECT/CT images were qualitatively analysed and compared with previously obtained microPET/CT images from our group using [18F] SF6 (1). Result(s) The designed system was shown to be amenable for NG-eTcM administration to rats. MicroSPECT/CT images were properly acquired, and they showed the radiopharmaceutical was able to reach the lungs. The system can easily be modified for studies in mice. When compared to microPET/CT images with [18F] SF6, the distribution pattern is much less homogeneous, probably due to the different nature of NG-eTcM nanoparticle suspension, not optimized for animal use, vs. a pure gas. Conclusion(s) The commercially available administration system of TechnegasTM has been modified for its use in preclinical studies. NG-eTcM can properly be delivered to rats by inhalation, allowing MicroSPECT/ CT studies in animal models of diseases producing altered lung ventilation patterns.
Keywords

Full text: Available Collection: Databases of international organizations Database: EMBASE Language: English Journal: European Journal of Nuclear Medicine and Molecular Imaging Year: 2022 Document Type: Article

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: Databases of international organizations Database: EMBASE Language: English Journal: European Journal of Nuclear Medicine and Molecular Imaging Year: 2022 Document Type: Article