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Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus.
Bertolini, Amanda Bezerra; Thyssen, Patrícia Jacqueline; Brandão, Paulo Eduardo; Prado, Aline Marrara; Silva, Sheila Oliveira de Souza; Mioni, Mateus de Souza Ribeiro; de Gouvea, Fábio Lucas Rezende; Pantoja, José Carlos de Figueiredo; Langoni, Helio; Guimarães, Felipe Freitas; Joaquim, Sâmea Fernandes; Guerra, Simony Trevizan; Leite, Domingos da Silva; Rall, Vera Mores; Hernandes, Rodrigo Tavanelli; Lucheis, Simone Baldini; Rossi, Gabriel Augusto Marques; Ribeiro, Márcio Garcia.
  • Bertolini AB; Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP, 18618-681 Botucatu, SP, Brazil.
  • Thyssen PJ; Department of Animal Biology, Institute of Biology, University of Campinas-UNICAMP, Campinas, 13083-862 SP, Brazil.
  • Brandão PE; Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of São Paulo, FMVZ-USP, 05508-900 São Paulo, SP, Brazil.
  • Prado AM; Department of Animal Biology, Institute of Biology, University of Campinas-UNICAMP, Campinas, 13083-862 SP, Brazil.
  • Silva SOS; Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of São Paulo, FMVZ-USP, 05508-900 São Paulo, SP, Brazil.
  • Mioni MSR; Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP, 18618-681 Botucatu, SP, Brazil.
  • de Gouvea FLR; Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP, 18618-681 Botucatu, SP, Brazil.
  • Pantoja JCF; Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP, 18618-681 Botucatu, SP, Brazil.
  • Langoni H; Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP, 18618-681 Botucatu, SP, Brazil.
  • Guimarães FF; Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP, 18618-681 Botucatu, SP, Brazil.
  • Joaquim SF; Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP, 18618-681 Botucatu, SP, Brazil.
  • Guerra ST; Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP, 18618-681 Botucatu, SP, Brazil.
  • Leite DDS; Department of Genetics, Evolution, Microbiology, and Immunology, UNICAMP, 13083-862 Campinas, SP, Brazil.
  • Rall VM; Department of Chemical and Biological Sciences, Institute of Biosciences, UNESP, 18618-689 Botucatu, SP, Brazil.
  • Hernandes RT; Department of Chemical and Biological Sciences, Institute of Biosciences, UNESP, 18618-689 Botucatu, SP, Brazil.
  • Lucheis SB; Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP, 18618-681 Botucatu, SP, Brazil.
  • Rossi GAM; Vila Velha University (UVV), Faculty of Veterinary Medicine, Department of Veterinary Medicine, 29102-920 Vila Velha, ES, Brazil.
  • Ribeiro MG; Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Sciences, São Paulo State University-UNESP, 18618-681 Botucatu, SP, Brazil.
J Appl Microbiol ; 134(3)2023 Mar 01.
Article in English | MEDLINE | ID: covidwho-2222665
ABSTRACT

AIMS:

We aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms. METHODS AND

RESULTS:

We collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae (152/217 = 70%) 11 families of Diptera were identified. Rotavirus A (RVA) and betacoronavirus (BCoV) were detected in 14.7% (32/217) and 4.6% (10/217) of the dipterans, respectively. Sequencing of the amplicons was possible for 11.5% (25/217) of RVA and 0.5% (1/217) of BCoV, confirming the presence of these pathogens.

CONCLUSIONS:

Our findings highlight the role of dipterans as carriers of RVA and BCoV of great relevance for public and animal health.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Rotavirus Infections / Cattle Diseases / Rotavirus / Diptera Type of study: Observational study / Randomized controlled trials Limits: Animals Language: English Journal subject: Microbiology Year: 2023 Document Type: Article Affiliation country: Jambio

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Rotavirus Infections / Cattle Diseases / Rotavirus / Diptera Type of study: Observational study / Randomized controlled trials Limits: Animals Language: English Journal subject: Microbiology Year: 2023 Document Type: Article Affiliation country: Jambio